| HPLC法同时测定川芎药材中阿魏酸和藁本内酯的含量 |
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| 作者姓名： | 吕维 吴恋 叶娜 等 |
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| 作者单位： | 成都中医药大学药学院 |
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| 摘 要： | 目的:建立高效液相色谱法同时测定川芎药材中阿魏酸和藁本内酯的方法。方法:采用Dikma Kromasil C18(250 nm×4.6 mm,5μm)色谱柱;流动相为甲醇-0.1%冰醋酸水溶液(65∶35);流速1.0 mL.min-1;检测波长:320nm;柱温30℃。结果:川芎药材中2种成分得到良好的分离,阿魏酸在0.032~0.3179μg范围内,藁本内酯在0.244~2.44μg范围内线性关系良好,平均回收率分别为98.4%和98.8%。结论:本方法简便,准确,重复性好,为多组分评价川芎药材质量提供了可靠的分析方法。
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| 关 键 词： | HPLC 阿魏酸 藁本内酯 川芎 |
Simultaneous determination of the content of ferulic acid and figustilide in Chuanxiong by HPLC |
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| Authors: | LV Wei WU Lian YE Na LUO Hong-li WANG Chun-yan WAN Li |
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| Institution: | (Pharmacy College, Chengdu University of Traditional Chinese Medicine; The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine; State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources Co-founded by Sichuan Province and MOST, Chengdu 611137, China) |
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| Abstract: | Objective： To develop a HPLC method for simultaneous determination of the content of ferulic acid and ligustilide in Chuanxiong. Method： The analysis was performed an a Kromasil C18 （250 nm×4.6 mm,5μm） column. The mobile phase consisted of methanol-0.1%glacial acetic acid（65：35）.The flow rate was 1.0 mL· min^-1. The detection wavelength was 320 nm and column temperature was 30℃. Result： The ferulic acid and ligustilide was separated perfectively. The standard curve was liner within the concentration range of 0.032μg～0.3179μg for ferulic acid and 0.244μg -2.4 μg for ligustilide. The recovery was 98.4% for ferulic acid and 98.8% for ligustilide, respectively. Conclusion： The method is simple, accurate and reproducible, which can be used for the quality control of Chuanxiong. |
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| Keywords: | HPLC ferulic acid ligustilide Chuanxiong |
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