| 反相HPLC法测定药用人参中多种人参皂甙的含量 |
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| 引用本文： | 朱萱萱,茅泳雯,何润霞,正山征洋,山本惠一. 反相HPLC法测定药用人参中多种人参皂甙的含量[J]. 中国生化药物杂志, 1998, 0(1) |
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| 作者姓名： | 朱萱萱 茅泳雯 何润霞 正山征洋 山本惠一 |
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| 作者单位： | 南京中医药大学附属医院!210029(朱萱萱)，江苏省职工医科大学(茅泳雯)，南京生物化学制药研究所(何润霞)，日本九洲大学药学部(正山征洋，山本惠一) |
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| 摘 要： | 采用HPLC法对不同产地药用人参中多种人参皂甙（GinsenosideRf、Re、Rd、Rb1、Rb2、Rg1、Rg2、Re）进行定量分析，流动相分别选用乙腈-0．05mol／L磷酸二氢钠（30∶70）和（20∶80），流速1ml／min，紫外检测波长203nm，色谱柱：YMCParkA-312ODS（6min×150mmid），柱温35℃，回收率均≥96.90％，RSD＜0.56％。该法操作简便，精密度高，重视性好，为评价药用人参的品质提供了参考依据。
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| 关 键 词： | 药用人参 反相HPLC 人参皂甙 |
Determination of Ginsenosides in Panax Ginseng by HPLC |
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| Zhu Xuanxuan, Mao Yongwen, He Runxia, Y SHOYAMA, A YAMAMOTO. Determination of Ginsenosides in Panax Ginseng by HPLC[J]. Chinese Journal of Biochemical Pharmaceutics, 1998, 0(1) |
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| Authors: | Zhu Xuanxuan Mao Yongwen He Runxia Y SHOYAMA A YAMAMOTO |
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| Affiliation: | Zhu Xuanxuan; Mao Yongwen; He Runxia; Y SHOYAMA; A YAMAMOTO(Hospital of Traditional Chinese Medicine of Jiangsu Province (Nanjing 210029 |
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| Abstract: | To develop a HPLC analytical method for Ginsenosides in Panax Ginseng from different producing areas. Chromatographic conditions included column YMC Park A 312 ODS, UV detector (203 nm ), mobile phase CH3CN-0. 05 mol/L NaH2PO4 (30: 70) and CH3CN-0. 05 mol/L NaH2PO4(20: 80 ) respectively, column temperature (35 ℃ ), injecting volume (20μl ). The external standard curve was linear in the concentration range of 20~ 140 μg/ml and the correlation coefficient was larger than 0. 999 6. The relative standard differences (RSD) were less than 0. 79%. The method showed a good reproducibility. Favorable stability and high sensitivity were noted. This method was found to be useful for quality control of Panax Ginang. |
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| Keywords: | HPLC Ginsenosides Panax Ginseng |
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