High prevalence of edin-C encoding RhoA-targeting toxin in clinical isolates of Staphylococcus aureus |
| |
Authors: | P Munro R Clément J-P Lavigne C Pulcini E Lemichez and L Landraud |
| |
Institution: | (1) INSERM, U895, C3M, Toxines Microbiennes dans la Relation H?te Pathog?nes, Universit? de Nice-Sophia-Antipolis, UFR M?decine, IFR50, Nice, 06204, France;(2) Universit? de Nice-Sophia-Antipolis, UFR M?decine, IFR50, Nice, 06204, France;(3) Service d’Infectiologie, H?pital l’Archet 1, Route Saint Antoine de Ginesti?re, BP 3079, 06202 Nice Cedex 3, France;(4) INSERM, Espri 26, Universit? Montpellier 1, UFR de M?decine, N?mes, 30908, France;(5) Laboratoire de Bact?riologie, CHU Caremeau, N?mes, 30908, France;(6) Laboratoire de Bact?riologie, CHU de Nice, H?pital l’Archet, Nice, 06202, France; |
| |
Abstract: | Staphylococcus aureus, a major causative agent of human infection, produces a large array of virulence factors, including various toxins. Among
them, the host RhoA GTPase ADP-ribosylating EDIN toxins are considered as potential virulence factors. Using the polymerase
chain reaction (PCR) assay, we analyzed the virulence profile of 256 S. aureus isolates from various clinical sites of infections. We developed specific primers to detect the three isoforms of edin-encoding genes. We found a prevalence of 14% (36 bacteria) of edin-encoding genes among these clinical isolates. Strikingly, we found that 90% of all edin-bearing S. aureus isolates carried the type-C allele. Both the spa types and the profile of virulence factors of these edin-positive isolates are highly variable. Notably, we show for the first time that edin-C-positive isolates were more frequently recovered from deep-seated infections than other types of infections. Our present
work, thus, strongly suggests that the presence of edin-C is a risk factor of S. aureus dissemination in tissues and, thus, represents a predictive marker for a pejorative evolution of staphylococcal infections. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|