Detection and full genomic analysis of G6P[9] human rotavirus in Japan |
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Authors: | Dai Yamamoto Mitsuyo Kawaguchiya Souvik Ghosh Maho Ichikawa Kei Numazaki Nobumichi Kobayashi |
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Institution: | (1) Departments of Hygiene and Pediatrics, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo 060-8556, Japan;(2) Department of Hygiene, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo 060-8556, Japan;(3) Department of Pediatrics, International University of Health and Welfare, Tochigi, Japan |
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Abstract: | A rare genotype G6P9] was identified in two human group A rotavirus strains designated as KF14 and KF17, that were detected
in stool specimens from children with diarrhea in Japan. VP7 gene sequences of these two strains were identical and genetically
closely related to G6 human rotavirus strains reported in European countries and the United States. To our knowledge, this
is the first report of detection of a G6 human rotavirus in Japan. For further genetic analysis to elucidate the origin of
the G6 rotavirus, nearly full-length sequences of all 11 RNA segments were determined for the KF17 strain. The complete genomic
constellation of KF17 was determined as G6-P9]-I2-R2-C2-M2-A3-N2-T3-E3-H3, a novel genotype constellation for human rotavirus.
Phylogenetic analysis indicated that VP6, VP1-3, and NSP2 genes of KF17 clustered with bovine-like G6 human strains and some
animal strains into sub-lineages distinct from those of common DS-1-like G2 human rotaviruses. On the other hand, KF17 genes
encoding VP4, NSP1, and NSP3-5 showed high sequence identities to the human G3P9] strain AU-1, and clustered with AU-1 and
some feline strains within the same lineage. These findings suggested that the G6P9] human rotavirus detected in Japan may
have occurred through reassortment among uncommon bovine-like human rotaviruses and human/feline AU-1-like rotaviruses. |
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