原发性胰腺癌患者高强度聚焦超声治疗前后外周血CD4+CD25+调节性T细胞的变化及其意义

目的：探讨原发性胰腺癌患者接受高强度聚焦超声(HIFU)治疗前后外周血CD4+
CD25+调节性T细胞的变化,阐明HIFU治疗对原发性胰腺癌患者免疫功能的影响。方
法：原发性胰腺癌组患者36例，对照组体检健康者28例，于HIFU治疗前、治疗4周后分别抽取外周静脉血，采
用流式细胞术检测外周血中CD4+CD25+调节性T细胞的百分比，并比较治疗前后原发性胰
腺癌患者KPS评分和癌胚抗原（CEA）、糖类抗原199（CA-199）水平的变化。结果： HIFU治疗前原发性胰腺癌组患者
外周血CD4+ CD25+ 调节性T细胞百分比高于对照组,差异有统计学意义（P<0.01）；原发性胰腺癌组患者经HIFU治疗4周后外周血CD4+CD25+调节性T细胞百分比较治疗前明显下降,差异有统计学意义（P<0.01）。原发性胰腺癌组患者治疗后KPS评分与治疗前比较明显上升，差异有统计学意义（P<0.05）；CEA和CA-199水平与治疗前比较均明显下降，差异有统计学意义（P<0.05）。结论：HIFU治疗可以改善原发性胰腺癌患者的细胞免疫功能。

Changes of peripheral blood CD4 + CD25 + regulatory T cells in patients with primary pancreatic cancer before and after treatment of high intensity focused ultrasound and significance

Objective To investigate the changes of peripheral blood CD4+CD25+
regulatory T cells in patients with primary pancreatic cancer before and after
treatment of high intensity focused ultrasound(HIFU),and to elucidate the effects of HIFU treatment on immune fun
ction of the patients with primary pancreatic cancer. Methods
Flow cytometry was used to detect the percentage
of CD4+CD25+ regulatory T cells in peripheral blood of the patients
in pancreatic carcinoma group(n=36) before and 4 weeks after HIFU treatment and healthy persons in control group(n=28).The KPS scores,the levels of CEA
and CA-199 of the patients with primary pancreatic cancer before and after treatment were compared.
Results The percentage of CD4+CD25+ regulatory Tcells of the patients before HIFU treatment was significantly higher than that in control group,the difference was statistically significant (P<0.01).The percentage of CD4+CD25+ regulatory T cells of the patients in pancreatic cancer group 4 weeks after HIFU treatment was lower than before treatment,the difference wasstatistically significant (P<0.01).The KPS score of 36 patients with primary pancreatic cancer after HIFU treatment was significantly increased compared with before treatment,the difference was statistically significant ( P<0.05 ).The
levelsof CEA and CA-199 of the patients after HIFU treatment was significantly decreased compared with before treatment,the difference was statistically significant (P<0.05).Conclusion HIFU treatment can improve the cell immune function of the patients with pancreatic cancer.
