B7-H4蛋白在卵巢上皮性肿瘤组织中的表达及其临床意义

目的：研究B7-H4蛋白在卵巢上皮性肿瘤组织中的表达，探讨其与卵巢上皮性肿瘤临床病理学特征的关系及与CA125表达的相关性。方法： 采用免疫组织化学法检测B7-H4 蛋白和CA125 在70例卵巢上皮性癌（卵巢浆液性囊腺癌41例、卵巢黏液性囊腺癌11例、卵巢子宫内膜样癌9例和卵巢透明细胞癌9例）、30例卵巢良性上皮性肿瘤及10例正常卵巢组织中的表达。结果：① B7-H4蛋白在卵巢上皮性癌组织中的阳性表达率（84.3%）显著高于卵巢良性上皮性肿瘤（23.3%，P<0.05），在正常卵巢组织中呈阴性表达。② B7-H4蛋白在卵巢浆液性囊腺癌、卵巢子宫内膜样癌和卵巢透明细胞癌组织中的阳性表达率（100%、77.8%和88.9%）均显著高于卵巢黏液性囊腺癌（27.3%，P<0.01）。③ B7-H4蛋白在卵巢上皮性癌晚期组（Ⅲ和Ⅳ期）的阳性表达率（92.9%）显著高于卵巢上皮性癌早期组（Ⅰ和Ⅱ期）（71.4%，P<0.05)。④ B7-H4蛋白在卵巢上皮性癌低分化组（G3）的阳性表达率（100%）显著高于卵巢上皮性癌高、中分化组（G1和G2）（67.6%，P<0.01）。⑤除卵巢黏液性囊腺癌外，CA125在卵巢黏液性癌、卵巢子宫内膜样癌和卵巢透明细胞癌组织中的阳性表达率均显著低于B7-H4的阳性表达率，CA125与B7-H4表达率呈正相关关系（r=0.277，P<0.05）。结论：B7-H4蛋白在卵巢上皮性癌组织中的阳性表达与卵巢上皮性癌的病理类型、临床分期和病理分级相关，B7-H4可以作为诊断卵巢癌新的肿瘤标志物之一。

Expression of B7-H4 protein in ovarian epithelial tumor tissue and its clinical significance

Objective To study the expression of B7-H4 protein in ovarian epithelial tumor tissue and to explore its association with the clinical pathologicalcharacteristics of ovarian epithelial tumor and the relationship between the expressions of CA125 and B7-H4.Methods The immunohistochemistry method was used to detect the expressions of B7-H4 and CA125 in 70 patients with ovarian epithelial carcinoma including 41 cases of ovarian serous cystadenocarcinoma,11 cases of ovarian mucous cystadenocarcinoma,9 cases of ovarian endometrioid carcinoma,9 cases of ovarian clear cell carcinoma,30 cases of ovarian benign epithelial tumor and 10 cases of normal ovary tissue.Results ① The positive expression rate of B7-H4 in ovarian epithelial carcinoma(84.3%) was significantly higher than that in ovarian benign epithelial tumor (23.3%,P<0.01),and the B7-H4 expression was negative in normal ovary tissue.② The positive expression rates of B7-H4in ovarian serous cystadenocarcinoma (100.0%),ovarian endometrioid carcinoma (77.8%) and ovarian clear cell carcinoma (88.9%) were significantly higher thanthat in ovarian mucus cystadenocarcinoma (27.3%,P<0.05).The positive expression rate of B7 - H4 in advanced epithelial ovarian carcinoma group (Ⅲ period and Ⅳ period) (92.9%) was statistically higher than that in early epithelial ovarian carcinoma group (Ⅰ period and Ⅱ period) (71.4%,P<0.05).④ The positive expression rate of B7-H4 in low differentiation (G3) ovarian epithelial carcinoma group (100%) was statistically higher than that in high-medium differentiation (G1 and G2) ovarian epithelial carcinoma group (67.6%,P<0.01).⑤Thepositive expression rates of CA125 in ovarian serous cystadenocarcinoma,ovarian mucinous carcinoma,ovarian endometrioid carcinoma,ovarian clear cell carcinoma tissue,excluding ovarian mucous cystadenocarcinoma,were lower than those of B7-H4,and they were positively correlated (r=0.277,P<0.05).Conclusion The positive expression rate of B7-H4 in ovarian epithelial carcinomatissue is related to the pathological type,clinical stage and pathological grade of ovarian epithelial carcinoma.B7-H4 may be regarded asa new marker used for diagnosis of ovarian cancer.