| DTT诱导P38核转位与食管癌Eca109细胞凋亡的研究 |
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| 引用本文： | 冯若,刘国红,翟文龙,金辉,张钦宪.DTT诱导P38核转位与食管癌Eca109细胞凋亡的研究[J].河南职工医学院学报,2008,20(2):107-109. |
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| 作者姓名： | 冯若 刘国红 翟文龙 金辉 张钦宪 |
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| 作者单位： | 1. 郑州大学医学院组胚教研室
2. 郑州大学第一附属医院普外科,河南,郑州450052 |
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| 摘 要： | 目的探讨P38的核转位与DTT诱导的食管癌细胞凋亡的关系。方法采用DTT处理食管癌Eca109细胞，未加药组作为对照。流式细胞仪技术检测细胞凋亡率；免疫组化方法检测P38的磷酸化水平及磷酸化P38的核转位情况。结果与对照组相比，DTT处理组细胞凋亡率明显升高。免疫组化结果显示：处理组P38的磷酸化水平明显高于对照组（P〈0．01），且处理组P—P38大部分定位于核内。结论P—P38核转位参与了DTT诱导的食管癌Eca109细胞凋亡。
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| 关 键 词： | P38 核转位 凋亡 DTT Eca109细胞系 |
Study on DTT Induced Nuclear Translocation of P38 and Apoptosis of the Esophageal Carcinoma Cell Line Eca109 |
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| FENG Ruo,LIU Guo-hong,ZHAI Wen-long,et al..Study on DTT Induced Nuclear Translocation of P38 and Apoptosis of the Esophageal Carcinoma Cell Line Eca109[J].Journal of Henan Medical College For Staff and Workers,2008,20(2):107-109. |
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| Authors: | FENG Ruo LIU Guo-hong ZHAI Wen-long |
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| Institution: | FENG Ruo1,LIU Guo-hong1,ZHAI Wen-long2,et al.(1.Department of Histology , Embryology,Medical College of Zhengzhou University,Zhengzhou Henan2.Department of General Surgery,the First Affiliated Hospital of Zhengzhou University,Zhengzhou Henan 450052 China) |
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| Abstract: | Objective To study the relationship between nuclear translocation of p-P38(phosphorylated P38) and apoptosis induced by DTT.Methods Esophageal carcinoma cell line Eca109 was treated with DTT.The untreated cells served as the control group.The esophageal carcinoma cell apoptosis was detected by flow cytometry.The P38 phosphorylation was detected by immunohistochmestry.Results The apoptotic rate of DTT treated group was higher than that of control group(P<0.01).The phosphorylation level of the treatment group... |
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| Keywords: | nuclear translocation apoptosis DTT Eca109 cell line |
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