| 氟化钠对原代培养大鼠肝细胞的毒性作用 |
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| 引用本文： | 郭晓英,李革新,孙贵范,孙迎春.氟化钠对原代培养大鼠肝细胞的毒性作用[J].中国公共卫生,2004,20(10):1192-1193. |
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| 作者姓名： | 郭晓英 李革新 孙贵范 孙迎春 |
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| 作者单位： | 1. 中国医科大学公共卫生学院流行病学教研室,沈阳,110001
2. 沈阳市疾病预防控制中心 |
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| 摘 要： | 目的 探讨氟化钠 (NaF)对原代培养大鼠肝细胞的毒性作用。方法 采用原代培养的方法 ,观察氟化钠对原代培养大鼠肝细胞存活率和肝细胞培养液中谷草转氨酶 (AST)和谷丙转氨酶 (ALT)活性的影响。结果 氟化钠可使原代培养大鼠肝细胞存活率下降 ,且呈现明显的剂量 -效应关系 ,其IC50 为 3 5 8mmol/L ;肝细胞培养液中ALT和AST的活性随染毒剂量的增加而逐渐升高 ,2和 4mmol/L染氟组肝细胞培养液中ALT和AST的活性与对照组相比显著升高 (P <0 0 5 )。结论 氟化钠对原代培养大鼠肝细胞有明显的毒作用。
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| 关 键 词： | 氟化钠 原代培养 肝细胞 |
| 文章编号： | 1001-0580(2004)10-1192-02 |
| 修稿时间： | 2004年2月1日 |
Cytotoxicity induced by sodium fluoride in primary cultured rat hepatocytes |
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| GUO Xiao-ying,LI Ge-xin,SUN Gui-fan,et al..Cytotoxicity induced by sodium fluoride in primary cultured rat hepatocytes[J].Chinese Journal of Public Health,2004,20(10):1192-1193. |
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| Authors: | GUO Xiao-ying LI Ge-xin SUN Gui-fan |
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| Institution: | GUO Xiao-ying,LI Ge-xin,SUN Gui-fan,et al.Department of Ep idemiology,School of Public Health,China Medical University |
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| Abstract: | Objective The cytotoxicity induced by sodium fluoride in primary cultured rat hepatocytes was studied.Methods Hepatocytes were cultured with various concentrat ions of sodium fluoride for 24 hours to observe its effect on the cell viability and the activities of AST and ALT.Results A dose-dependent decrease of cell viability was d etected in the hepatocytes and the IC\-\{50\} was 3.58?mmol/L.The activities of AST and ALT increased with the doses increase of sodium fluoride.The activit ies of AST and ALT in the 2 and 4?mmol/L groups were significantly high er than the control group(P<0.05).Conclusion Sodium fluoride significantly induced toxicity in primary cultured rat hepatocytes. |
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| Keywords: | sodium fluoride primary culture hepatocytes |
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