TFF2在幽门螺杆菌感染胃体部黏膜中的表达

目的:探讨三叶因子2(TFF2)在幽门螺杆菌(H pylori)感染胃体腺黏膜中表达的意义.方法:选择有H pylori感染的胃手术切除标本29例(92个蜡块),其中胃及十二指肠溃疡5例,胃癌24例,用免疫组织化学方法检测TFF2的分布和表达.结果:在H pylori感染胃黏膜中,TFF2主要在胃体腺的颈黏液细胞和假幽门腺化生细胞中表达;在正常黏膜中TFF2主要分布在胃固有腺的上1/3-2/3;在轻度萎缩黏膜中,腺底部仅见少量阳性细胞,随着萎缩程度的加重,在腺底部出现的阳性细胞增多;在轻、中度萎缩黏膜中其强阳性率分别为18.0%和42.9%,而在重度萎缩中达90.5%.在轻、中度和重度萎缩之间有显著性差异(P<0.01).结论:H pylori感染增加在腺底部的TFF2表达,并与黏膜的萎缩程度有关.

Expression of trefoil factor family 2 in fundic glands with Helicobacter pylori infection

AIM:To investigate the protein expression of the trefoil factor family 2(TFF2)in fundic glands with Helicobacter pylori infection. METHODS:Protein expression of TFF2 was detected by immunohistochemistry in paraffin- embedded samples from 29(92 blocks)surgical specimens from five patients gastric ulcer and 24 with gastric carcinoma. RESULTS:Expression of TFF2 was found in mucous neck cells and cells with pseudopylori gland metaplasia.Positive cells were distributed above the 1/2 to 2/3 portions of fundic glands in normal gastric mucosa.The ratio of TFF2- positive cells in the basal portion of the fundic glands was 18.0,42.9 and 90.5% in mild,moder- ate and severe glandular atrophy,respectively, and these differences were significant(P<0.01). CONCLUSION:H pylori infection increases the expression of TFF2 in deeper parts of the fundic gland,and is also associated with the degree of glandular atrophy.The expression of TFF2 is re- lated to mucosal protection and repair processes.