Exendin-4对2型糖尿病大鼠肾组织MMP-9与TIMP-1表达的影响

目的观察Exendin-4对2型糖尿病大鼠肾组织基质金属蛋白酶9(MMP-9)、金属蛋白酶组织抑制物1(TIMP-1)表达的影响。方法建立2型糖尿病大鼠模型后,分为糖尿病组,exendin-4低、中、高剂量组。给药6周后,采用免疫组织化学方法检测各组大鼠肾组织中MMP-9、TIMP-1的蛋白表达。结果糖尿病组MMP-9、TIMP-1的表达较正常对照组明显增强(P<0.01);exendin-4各组较糖尿病组MMP-9、TIMP-1表达减弱(P均<0.01)。结论 Exendin-4对2型糖尿病大鼠有一定的肾脏保护作用,其机制可能部分是通过下调2型糖尿病大鼠肾组织MMP-9和TIMP-1的表达来实现的。

Effects of Exendin-4 on Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Renal Tissue of Type 2 Diabetic Rats

Objective To observe the effects of exendin-4 on the expression of matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in the renal tissue of type 2 diabetic rats. Methods The diabetic rats were divided into 4 groups： diabetes mellitus group（DM group）, low dose of exendin-4 group（EL group）, middle dose of exendin-4 group（EM group）, high dose of exendin-4 group（EH group）. After 6 weeks, the expression of MMP-9 and TIMP-1 in renal tissue was measured by immunohistochemistry. Results The expression of MMP-9 and TIMP-1 in DM group was higher than that in NC group（P〈0 .01）, while the expression of MMP-9 and TIMP-1 in all doses of exendin-4 groups was lower than that in DM group（P〈0 .01）.Conclusion Exendin-4 has some renoprotective effect on type 2 diabetic rats, partly through down-regulating the expression of MMP-9 and TIMP-1.