CD80 expression is decreased in hyperplastic lymph nodes of HIV+ patients |
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Authors: | Legendre C; Raphael M; Gras G; Lefevre EA; Feuillard J; Dormont D; Richard Y |
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Institution: | CEA, Service de Neurovirologie, DSV/DRM, CRSSA, IPSC, Fontenay aux Roses, France. |
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Abstract: | A centrofollicular hyperplasia is present within secondary lymphoid organs
during all the asymptomatic phase of the HIV disease. Although this
hyperplasia has been well characterized by histological studies, the nature
of the phenotypic alterations in B cell populations occurring within HIV+
lymphoid organs remains to be established. By immunohistochemistry, we thus
investigated whether a particular germinal center (GC) B cell population
was increased during HIV-induced hyperplasia and whether any phenotypic
change was specific to HIV-1 infection. As compared to normal tonsils
(three cases) and HIV- hyperplastic lymph nodes (eight patients), we
observed a loss of GC polarization in all HIV+ sections (11 patients), with
no more delineation between dark and light zones, as shown by Ki67, CD10,
CD77, CD95 and CD86 staining. In contrast to CD86 expression which remained
as intensive in HIV+ as in HIV- lymph nodes, CD80 staining was strongly
decreased in GC of HIV+ lymph nodes but not in their extrafollicular zones.
The loss of CD80 expression from CD19+ B cells was also observed by
cytometric analysis of cell suspensions of three HIV+ patients. Although we
found no evidence of an increase in a particular GC B cell subset in
HIV-1-induced hyperplasia, the strong GC disorganization observed may
induce impaired cell-cell interactions and thus participate in the loss of
CD80 antigen. In contrast to HIV- situations where CD80 and CD86 was
similarly expressed on B cells, the lower level of CD80 expression in HIV+
GC may favor Th2 T cell responses through CD86-CD28 interactions.
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