| 血管内皮生长因子在妊娠小鼠子宫GMG细胞中的表达 |
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| 引用本文： | 魏静波,翁静. 血管内皮生长因子在妊娠小鼠子宫GMG细胞中的表达[J]. 首都医科大学学报, 2007, 28(4): 476-478 |
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| 作者姓名： | 魏静波 翁静 |
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| 作者单位： | 首都医科大学基础医学院组织胚胎学教研室;首都医科大学基础医学院组织胚胎学教研室 |
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| 基金项目： | 首都医科大学校科研和校改项目;北京市优秀人才培养基金 |
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| 摘 要： | 目的探讨颗粒子宫腺(GMG)细胞中血管内皮生长因子(VEGF)的表达。方法取妊娠13~15 d昆明种小鼠的子宫腺细胞区,经组织块培养提纯GMG细胞,应用免疫组织化学及Western blot法显示GMG细胞中VEGF。结果组织块培养时GMG细胞生长状况好,细胞纯度高,在GMG细胞及其培养液中均有VEGF表达。结论GMG细胞表达和分泌VEGF。
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| 关 键 词： | 颗粒子宫腺细胞 血管内皮生长因子 |
| 收稿时间： | 2006-06-14 |
| 修稿时间： | 2006-06-14 |
Expression of Vascular Endothelial Growth Factor in the Granulated Metrial Gland Cells |
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| Wei Jingbo,Weng Jing. Expression of Vascular Endothelial Growth Factor in the Granulated Metrial Gland Cells[J]. Journal of Capital Medical University, 2007, 28(4): 476-478 |
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| Authors: | Wei Jingbo Weng Jing |
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| Affiliation: | Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University |
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| Abstract: | Objective To investigate the expression of vascular endothelial growth factor(VEGF) in the granulated metrial gland(GMG) cells.Methods Metrial glands were taken from pregnant Kunming mice at 13～15th gravid day.GMG cells were got and purified by tissue culture.Expression of VEGF in the GMG cells was shown with immunohistochemical staining and Western blot.Results GMG cells migrated from the tissue culture blots and grew well.Expression of VEGF was shown in these cells by immunohistochemical staining and Western blot.Moreover VEGF was also shown in GMG cells culture medium.Conclusion The GMG cells can express and excrete VEGF. |
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| Keywords: | GMG cells VEGF |
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