| SARS冠状病毒S蛋白受体结合域的克隆测序 |
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| 引用本文： | 高俊,肖洪广,林勇平. SARS冠状病毒S蛋白受体结合域的克隆测序[J]. 广州医药, 2006, 37(5): 49-52 |
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| 作者姓名： | 高俊 肖洪广 林勇平 |
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| 作者单位： | 广州医学院第一附属医院检验科,广州,510120;广州医学院第一附属医院检验科,广州,510120;广州医学院第一附属医院检验科,广州,510120 |
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| 基金项目： | 广东省中山市卫生局资助项目 , 广东省广州市教委科研项目 |
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| 摘 要： | 目的 从来源于人和果子狸的SARS冠状病毒S蛋白基因中,获得受体结合域(RBD)基因的克隆.方法 用PCR方法扩增RBD基因,将其克隆到pGEM-T Easy载体,转化后挑取阳性克隆进行酶切和测序鉴定,并分析RBD基因序列.结果 获得了人和果子狸来源的RBD的基因片段,长度为579 bp,两者具有高度的同源性.结果 RBD是SARS冠状病毒与靶细胞结合的部位,本工作成功克隆了SARS冠状病毒的RBD基因,为该基因的表达和功能研究奠定了基础.
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| 关 键 词： | SARS冠状病毒 受体结合域 克隆 测序 |
| 收稿时间： | 2006-06-15 |
| 修稿时间： | 2006-06-15 |
Cloning and sequence analysis of receptor binding domain on the SARS-CoV spike protein |
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| Gao Jun,Xiao Hongguang,Lin Yong ping. Cloning and sequence analysis of receptor binding domain on the SARS-CoV spike protein[J]. Guangzhou Medical Journal, 2006, 37(5): 49-52 |
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| Authors: | Gao Jun Xiao Hongguang Lin Yong ping |
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| Affiliation: | Laboratory Department, the First Affiliated Hospital of Guangzhou Medical College, Guangzhou, 510120 |
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| Abstract: | Objective To sequence and analyze receptor binding domain(RBD) fragment on SARS-CoV Spike Protein form humans and palm civet.Methods RBD genes were amplified by PCR assay.then RBD segments were identified with restriction enzyme digestion and sequence after beening ligased into pGEM-T Easy vector.Results The RBD genes from two species were successfully cloned which were 579bp in length and high homologous.Conclusion The clone of RBD gene provide the basis of further study on expression and function research of RBD. |
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| Keywords: | SARS-CoV Receptor binding domain(RBD) Clone sequence |
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