The mycotoxin ochratoxin A inhibits DNA topoisomerase II and induces polyploidy in cultured CHO cells |
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| Authors: | Simona Cosimi, Luis Orta, Santiago Mateos,Felipe Cort s |
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| Affiliation: | aDepartment of Cell Biology, Faculty of Biology, University of Seville, Av. Reina Mercedes 6, E-41012 Seville, Spain |
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| Abstract: | Ochratoxin A (OTA), a known nephrotoxin and carcinogenic mycotoxin, was investigated to examine its effectiveness to induce cytotoxicity and DNA damage (Comet assay), as well as its possible inhibition of topoisomerase II (topo II) catalytic activity in cultured Chinese hamster ovary (CHO) cells. The analysis of OTA-induced DNA strand breaks as well as the flow cytometric assessment of polyploidy has provided evidence that is consistent with the idea of a mixed mode of action of the mycotoxin: in addition to its genotoxic activity, OTA may also interfere with chromosome distribution during cell division. |
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| Keywords: | Ochratoxin A Cytotoxicity DNA damage Topoisomerase II Polyploidy |
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