出口梗阻性便秘对大鼠血浆胃动素和血管活性肠肽含量的影响

目的研究出口梗阻性便秘对大鼠血浆胃动素（MTL）和血管活性肠肽（VIP）含量的影响。方法采用直肠部分缩窄法建立出口梗阻性便秘的动物模型，通过体外拆线解除梗阻，采用放射免疫分析法检测血浆MTL和VIP含量。结果模型组大鼠结肠黏膜显著水肿充血，大量炎症细胞浸润。模型组大鼠血浆MTL水平与正常组、解扎组大鼠差异无统计学意义，但其血浆VIP含量显著低于正常组（P〈0．01），与解扎组差异无统计学意义。结论出口梗阻性便秘导致大鼠结肠黏膜显著的炎症反应，也影响血浆VIP水平。

Effect of Constipation by Outlet Obstruction on the Content of Plasm Motilin and Vasoactive Intestinal Peptide of Rats

Objective To study the alteration of plasm motilin and vasoactive intestinal peptide of rats with constipation by outlet obstruction. Methods The models of constipation by outlet obstructio were made by constricting the rectum of rats partly,and taking out stitches in vitro to relieve obstructio. The contents of plasma motilin and vasoactive intestinal peptide were measured by radio-immunity analyzing. Results The tunica mu-cosa coil of model group rats were hydropsia,and concomitance with inflammatory cell infiltration. There was no significant difference in the levels of plasma MTL in all groups, but the levels of plasma VIP of model group were significantly lower than normal group (P<0.01). There was no significant difference between model group and relieve obstructio group. Conclusion Constipation by outlet obstruction results in inflammation of tunica muco-sa coli of model group rats,and effects the levels of plasma VIP.