HPLC法测定穿心莲内酯滴丸中穿心莲内酯的含量 |
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引用本文: | 敬永升,胡海廷,张雨. HPLC法测定穿心莲内酯滴丸中穿心莲内酯的含量[J]. 河南大学学报(医学版), 2009, 28(3): 200-201,231 |
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作者姓名: | 敬永升 胡海廷 张雨 |
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作者单位: | 河南大学,药学院,河南,开封,475004;河南大学,药学院,河南,开封,475004;河南大学,药学院,河南,开封,475004 |
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摘 要: | 目的:探讨测定穿心莲内酯滴丸中穿心莲内酯含量的方法。方法:采用HPLC法测定,使用Hypersil-CN(150 mm×4.6 mm,5μm)色谱柱,以甲醇∶水(40∶60)为流动相,流速1.0 ml/min,检测波长254 nm。结果:穿心莲内酯在20.0~100.0μg/ml范围内有很好的线性关系,其线性方程为A=11484C+35642(n=5,r=0.9998),回收率为100.53%,RSD=0.39%(n=6)。结论:HPLC法测定穿心莲内酯滴丸中穿心莲内酯线性关系好、准确度高、稳定性好、专属性强,可作为该产品的定量控制方法。
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关 键 词: | HPLC法 滴丸 穿心莲内酯 |
Determination of the andrographolide in andrographolide pill by HPLC |
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Abstract: | Objective: To establish the determination method for the of andrographolide in andrographolide pill with HPLC.Methods: The HPLC method was used at a Hypersil-CN column(150mm×4.6mm,5μm) as analytical column,with the mobile phase of methanol-water(40:60).The flow rate was 1.0ml/min,and the detection wave-length was 254nm.Results: The concentration of andrographolide was linearly related to its peak area in the rang of 20.0~100.0 μg/ml,the linear regression equation was A=11484C+35642(n=5,r=0.9998).The recovery was 100.53% and RSD of 0.39%(n=6).Conclusion: The determination of andrographolide in andrographolide pill with HPLC has a good linear relation with the high accurate rate and the good stability,so it can be used for the quantitative analysis. |
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Keywords: | HPLC Andrographolide pill Andrographolide |
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