| 半枝莲提取物诱导结肠癌HT-29细胞凋亡的机制研究 |
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| 引用本文： | 彭军,林久茂,魏丽慧,徐伟.半枝莲提取物诱导结肠癌HT-29细胞凋亡的机制研究[J].福建中医学院学报,2010,20(6). |
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| 作者姓名： | 彭军 林久茂 魏丽慧 徐伟 |
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| 作者单位： | 1. 福建中医药大学中西医结合研究院,福建,福州,350108
2. 福建中医药大学药学院,福建,福州,350108 |
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| 基金项目： | 福建省自然科学基金，福建省中西医结合老年性疾病重点实验室开放课题 |
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| 摘 要： | 目的探讨半枝莲提取物在体外诱导结肠癌HT-29细胞株凋亡的作用机制。方法采用人结肠癌细胞HT-29常规体外培养,随机设定对照组和不同浓度半枝莲提取物组,干预24 h。倒置显微镜观察细胞形态变化,MTT法测定不同浓度半枝莲提取物对HT-29细胞增殖的影响,DNA Ladder试剂盒检测药物作用后细胞凋亡情况,AnnexinV-FITC/PI双标记流式细胞术检测细胞凋亡率,JC-1试剂盒检测线粒体膜电位变化,应用比色法分析Caspase-9和Caspase-3的活化情况。结果半枝莲提取物干预HT-29细胞24 h后,HT-29细胞密度明显减少、细胞变小,可明显抑制HT-29细胞增殖,细胞凋亡增加,线粒体膜电位丧失,具有显著地剂量依赖,并出现明显的DNA Lad-der条带;Caspase-9和Caspase-3的活化随着药物浓度的升高而增加。结论半枝莲提取物能通过线粒体通路诱导人结肠癌HT-29细胞凋亡而发挥其抗肿瘤作用。
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| 关 键 词： | 半枝莲 结肠癌 细胞凋亡 线粒体 |
Mechanism of Apoptosis of Human Colon Cancer HT-29 Cells Induced by Scutellaria barbata D.Don Extract |
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| PENG Jun,LIN Jiu-mao,WEI Li-hui,et al.Mechanism of Apoptosis of Human Colon Cancer HT-29 Cells Induced by Scutellaria barbata D.Don Extract[J].Journal of Fujian College of Traditional Chinese Medicine,2010,20(6). |
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| Authors: | PENG Jun LIN Jiu-mao WEI Li-hui |
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| Institution: | PENG Jun,LIN Jiu-mao,WEI Li-hui,et al(Fujian Academy of Integrative Medicine,FUTCM,Fuzhou,Fujian 350108,China) |
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| Abstract: | Objective To investigate the mechanism by which the extract of Scutellaria barbata D.Don(ESB) induces apoptosis of human colon carcinoma HT-29 cells.Methods Human colon carcinoma HT-29 cells were cultured in vitro and treated with different concentration of ESB for 24 hours.Cellular morphology of HT-29 cells was observed via phase-contrast microscopy.The cell viability was determined by MTT assay.Cell apoptosis was detected by DNA fragmentation and flow cytometry with Annexin V staining.Mitochondrial membra... |
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| Keywords: | HT-29 |
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