建立糖尿病心肌病动物模型方法的实验研究

目的研究建立糖尿病心肌病动物模型的科学、实用的方法。方法应用链脲佐菌素对SD大鼠进行一次性腹腔注射,通过观察大鼠的一般状况、进食量、进水量、尿量、体重的变化,监测血糖、尿糖、心肌酶含量、胰岛素水平、心肌超微结构、心脏大血管内膜情况及心电图情况来评估糖尿病心肌病的发病情况。结果腹腔注射STZ 3周后,模型组大鼠出现病态表现,进食量、饮水量、尿量增加,体重下降。血糖值、尿糖值及肌酸激酶、乳酸脱氢酶值均较对照组大鼠升高。胰岛素水平较对照组明显降低,心肌超微结构符合糖尿病心肌病表现,心脏大血管未见纤维斑块和粥样斑块。结论对SD大鼠进行一次性腹腔注射链脲佐菌素(55mg/kg),大鼠于注射后第3周可出现稳定的糖尿病心肌病状态。这是一种可靠且实用性强的糖尿病心肌病动物模型的制备方法。

A method to produce the animal model of diabetic cardiomyopathy

Objective To study a simple and scientific method to produce the animal model of diabetic cardiomyopathy.Methods Injecting the streptozotocin(STZ) liquid at the left iliac region only once in the model group,observing the general condition,the change of eating level,drinking level,urine level and weight,examining the level of creatine kinase(CK-MB),lactate dehydrogenase(LDH-1),insulin,blood sugar and urine sugar.Observe the cardiac muscle ultrastructure,the large vessel tunica intima and electrocardiogram.Results After injecting the STZ 3 weeks,the model rats appear morbid,the value of eating,drinking,urine are increased and weight decreased.The model rat blood sugar,urine sugar,CK and LDH levels are higher than control rat.The model rat insulin level is lower than control group.The model rat cardiac muscle ultrastructure accord with the diabetic cardiomyopathy's,and the fibrous or atheromatous plaque do not been found at the large vessel tunica intima.Conclusion The SD model rat appear the diabetic cardiomyopathy condition by injected the STZ(55mg/kg)at the left iliac region only once in 3 weeks.It is a simple and scientific method to produce the animal model of diabetic cardiomyopathy.