| 丹酚酸A对局灶性脑缺血再灌注损伤大鼠ICAM-1表达的影响 |
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| 引用本文： | 姜民,郭利平,周王怡,李晶,王辉. 丹酚酸A对局灶性脑缺血再灌注损伤大鼠ICAM-1表达的影响[J]. 辽宁中医杂志, 2009, 0(3): 469-471 |
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| 作者姓名： | 姜民 郭利平 周王怡 李晶 王辉 |
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| 作者单位： | 天津中医药大学; |
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| 基金项目： | 国家自然基金资助项目(30572444) |
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| 摘 要： | 目的:通过对观察丹酚酸A预处理对局灶性脑缺血再灌注损伤大鼠ICAM-1表达的影响,探讨丹酚酸A预处理对缺血再灌注急性期脑损伤的保护作用机制。方法:丹酚酸A预处理3天后采用线拴法建立大鼠脑缺血再灌注模型,不同时间点(0,1,4,12,24h)取脑组织,冰冻切片,荧光免疫组化法检测缺血区脑微血管内皮ICAM-1的表达。以尼莫地平作为阳性对照药。结果:模型组与空白对照组相比ICAM-1表达明显升高(P<0.05)。丹酚酸A各组ICAM-1表达均低于相应时间点模型组(P<0.05)。结论:丹酚酸A预处理可降低局灶性脑缺血再灌注损伤大鼠ICAM-1表达的影响,提示阻抑粒细胞粘附作用是其脑保护作用机制之一。
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| 关 键 词： | 丹酚酸A 缺血再灌注 ICAM-1 荧光免疫组化 |
Effect of Salvianolic Acid A in ICAM-1 Expression in Focal Brain Ischemia-reperfusion. |
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| JIANG Min,GUO Li-ping,ZHOU Wang-yi,LI Jing,WANG Hui. Effect of Salvianolic Acid A in ICAM-1 Expression in Focal Brain Ischemia-reperfusion.[J]. Liaoning Journal of Traditional Chinese Medicine, 2009, 0(3): 469-471 |
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| Authors: | JIANG Min GUO Li-ping ZHOU Wang-yi LI Jing WANG Hui |
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| Affiliation: | Tianjin University of Traditional Chinese Medicine;Tianjin 300193;China |
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| Abstract: | Objective:To study the effect of Salvianolic acid A in ICAM-1 expression in focal cerebra ischemia-reperfusion in rats.Methods:Establish focal cerebral ischemia reperfusion model in rats with suture method after pre-treated with Salvianolic acid A for 3 days.In different time courses(0,1,4,12,24 hours).Frozen section of brain tissue was detected by fluorescent-immunohistochemistry technique to test the expression of ICAM-1 on the microvascular endothelium of the ischemic brain region.Normal brain tissue was... |
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| Keywords: | Salvianolic acid A Ischemia-reperfusion ICAM-1 Fluorescent-immunohistochemistry |
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