| 对年轻细胞诱导早衰前后凋亡敏感性的研究 |
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| 引用本文： | 韩晓琳,张向阳,吴福国,张宗玉,童坦君.对年轻细胞诱导早衰前后凋亡敏感性的研究[J].济宁医学院学报,2004,27(4):14-17. |
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| 作者姓名： | 韩晓琳 张向阳 吴福国 张宗玉 童坦君 |
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| 作者单位： | 1. 济宁医学院生物化学教研室
2. 北京大学医学部生化与分子生物学系 |
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| 摘 要： | 目的探讨年轻细胞被诱导衰老后对凋亡的敏感性与年轻细胞的差异，并分析可能存在的机制。方法以人胚肺二倍体成纤维细胞(2BS)为研究对象，以H2O2诱导2BS细胞早衰后，再以H2O2分别诱导早衰2BS细胞和年轻细胞凋亡，DNA ladder分析和苔盼蓝排除法检测细胞死亡率。结果早衰细胞较年轻细胞不易被H202诱导凋亡。该因素是多方面的，不限于caspase-3的作用。结论早衰细胞与衰老细胞一样，对凋亡诱导因素的反应性降低。
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| 关 键 词： | 年轻 诱导凋亡 细胞诱导 敏感性 早衰 人胚肺二倍体成纤维细胞 caspase-3 BS 凋亡诱导 H202 |
THE STUDY ON THE SUSCEPTILITY TO APOPTOSIS OF YOUNG CELLS BEFORE AND AFTER BEING INDUCDE PREMATURE SENESCENCE |
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| Han Xiaolin,Zhang Xiangyang,Wu Fuguo,et al.THE STUDY ON THE SUSCEPTILITY TO APOPTOSIS OF YOUNG CELLS BEFORE AND AFTER BEING INDUCDE PREMATURE SENESCENCE[J].Jounal of Jinning Medical College,2004,27(4):14-17. |
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| Authors: | Han Xiaolin Zhang Xiangyang Wu Fuguo |
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| Abstract: | Objective To explore the difference susceptility to apoptosis of young cells before and after being induced senilism,and analysis the possible mechanism.Methods Human diploid fibroblasts are investigated in this study.After H 2O 2 was used to induce senilism in human diploid fibroblasts(strain 2BS),then to induce apoptosis in senilism cells and young ones.Apoptosis was detected by DNA ladder analysis and taipan blue excluding.Results The results showed that senilism cells are more resistant to apoptosis by H 2O 2 than young ones.The reasons is in many ways,it is not confined to function of caspase-3.Conclusion Senilism cells are sililar to serescent cells,reduce the susceptility to apoptosis. |
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| Keywords: | Senilism Cell apoptosis Fibroblasts(2BS) |
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