非小细胞肺癌患者外周血和骨髓中LUNX基因表达的临床意义

[目的]探讨非小细胞肺癌（NSCLC）患者外周血和骨髓微转移的肺特异性X蛋白（LUNX）基因诊断的临床意义.[方法]应用荧光定量逆转录聚合酶链反应（RT-PCR）技术,对42例肺癌患者和12例肺良性病变患者的外周血和骨髓中LUNX基因mRNA表达进行检测.[结果]42例肺癌患者有14例在外周血中检测到LUNX基因mRNA的表达,阳性表达率为33.3%,有10例在骨髓中检测到LUNX基因mRNA的表达,阳性表达率为23.8%.LUNX mRNA的表达与CK-19基因mRNA的表达率无显著性差异（P＞0.05）.外周血和骨髓微转移与肺癌组织学类型、细胞分化程度及TNM分期均存在密切关系（P＜0.05）.[结论]外周血和骨髓LUNX基因mRNA的表达是检测肺癌微转移的一个有价值的指标,为制定治疗方案和评估预后提供重要参考依据.

Lung-specific X Protein Expression in Patients with Non- small Cell Lung Cancer and Its Clinical Signifcance

[Purpose] To explore the clinical significance of lung-specific X protein(LUNX)gene for diagnosis to micrometastases in peripheral blood and bone marrow in non-small-cell lung cancer patients.[Methods] The LUNX mRNA expression was detected by TaqMan-based RT-PCR assays in peripheral blood and bone marrow from 42 cases with lung cancer and with 12 benign pulmonary lesion.[Results] The positive rates of LUNX mRNA in peripheral blood and bone marrow from lung cancer patients were 33.3 %(14/42)and 23.8 %(10/42)respectively.There was no significant difference between the positive rates of LUNX mRNA and that of CK-19 mRNA(P>0.05).The micrometastases in peripheral blood and bone marrow were closely related to the pathological type,cell differentiation and TNM stage(P<0.05).[Conclusions] LUNX mRNA might be a valuable marker for micrometastases in lung cancer patients,which may also provide important clinical information for prognositic evaluation and proper treatment planning.