紫杉醇衍生物GT对人喉癌细胞系Hep-2的临床价值分析

目的探讨紫杉醇衍生物GT对人喉癌细胞系Hep-2的临床价值。方法 GT对Hep-2细胞的增殖抑制作用应用MTT法进行测定,通过流式细胞术及细胞形态学考察GT对Hep-2细胞诱导凋亡的作用。结果在光镜下,GT作用细胞之后能观察到较为典型的细胞凋亡的形态学变化,如染色质断片化、细胞核固缩、核碎裂、染色质凝集成新月型紧贴于核膜周边、凋亡小体形成等。明显增加G2/M期的细胞比例,典型的亚二倍体"凋亡小峰"出现。结论紫杉醇衍生物GT能够明显抑制Hep-2细胞的生长,使细胞分裂阻滞于G2/M期,对肿瘤细胞凋亡有诱导作用,值得临床进一步对其进行研究开发。

Clinical Value of Taxol Derivative GT on Human Laryngeal Carcinoma Cell line Hep-2

Objective To investigate the clinical value of taxol derivative GT on human laryngeal carcinoma cell line Hep-2.Methods GT inhibited the proliferation of Hep-2 cell by MTT method were determined by morphology,flow cytometry and the effects of GT on apoptosis of Hep-2 cells induced by.Results Under light microscope,GT cells can be observed in the typical morphological changes of apoptosis,such as chromatin fragment,cell shrinkage,nuclear fragmentation,chromatin condensation integrated crescent clings to the nuclear periphery,the formation of apoptotic bodies.Increased G2／/M phase cells proportion,＂ typical hypodiploid apoptosis peak appeared small ＂.Conclusion Taxol derivative GT can obviously inhibit the growth of Hep-2 cells,the cell division arrest in G2／/M phase,in inducing the apoptosis of tumor cells,which is worthy of further research and development of the.