基质辅助激光解吸/电离飞行时间质谱在沙门菌检测和鉴定分型中的应用研究

目的利用基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)对从门诊腹泻病人粪便分离的88株沙门菌进行检测分型。方法利用优化的MALDI-TOF-MS实验条件对沙门菌进行检测和鉴定分型,并与血清学分型、耐药分型和PFGE分型结果进行比较。结果在反映亲缘关系的差异水平值为100时,88株沙门菌被分为15个MALDI-TOF-MS型别。MALDI-TOF-MS分型结合耐药表型分型,可以将88株沙门菌分成44个亚型;MALDI-TOF-MS分型结合血清学分型,可以将88株菌分成46个亚型。MALDI-TOF-MS分型结合PFGE分型,可以将88株菌分成64个亚型。说明MALDI-TOF-MS在沙门菌分型中具有很好的分型能力。结论MALDI-TOF-MS方法在检测与鉴定食源性病原菌中具有稳定、重复性好的特点,可成为食源性致病细菌分析鉴定的重要工具。

Study on detection and identification of Salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Objective To detect and type Salmonella species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).Methods With the optimized experimental conditions,a total of 88 Salmonella strains isolated from feces of patients suffered from diarrhea were applied onto MALDI-TOF-MS and the results were compared with those obtained from serotype profiles,drug-resistance profiles and PFGE profiles.Results The results illustrated that all strains tested were divided into 15 types by MALDI-TOF-MS at the distance level of 100.Eighty-eight strains were further break into 44 subtypes,based on the results of MALDI-TOF-MS combined with those of drug-resistance profiles.All strains were classified as 46 subtypes and 64 subtypes in combination MALDI-TOF-MS with serotype profiles and PFGE profiles,respectively.These reveal that MALDI-TOF-MS is a good method in Salmonella species typing.Conclusion The detection and identification of foodborne pathogenic bacteria by MALDI-TOF-MS is of good characteristics of stability and reproducibility.It is a powerful tool in detecting and identifying foodborne pathogenic bacteria.