| 高效液相色谱法测定丹红片中丹参素和原儿茶醛的含量 |
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| 引用本文： | 赵娅柯,张长弓,隆清娥,张梦洁,王腾.高效液相色谱法测定丹红片中丹参素和原儿茶醛的含量[J].中国药师,2012,15(1):68-70. |
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| 作者姓名： | 赵娅柯 张长弓 隆清娥 张梦洁 王腾 |
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| 作者单位： | 华中科技大学同济医学院药学院生药系 武汉430030 |
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| 摘 要： | 目的:建立丹红片中丹参素和原儿茶醛的含量测定方法.方法:采用高效液相色谱法,色谱柱为DiamonsilTM C18(250mm ×4.6mm,5μm),以甲醇-0.05％磷酸水溶液(20∶ 80)为流动相,检测波长为280 nm,流速为1.0 ml· min-1,柱温:30℃.结果:丹参素钠在8.160 ～97.920 mg·L-1的浓度范围内与其峰面积呈良好的线性关系(r=0.999 3),平均回收率为100.06％,RSD =0.46％(n=6)；原儿茶醛在0.292～ 2.336 mg·L-1范围内具有良好的线性关系(r =0.999 1),平均回收率为99.92％,RSD=0.69％(n=6).结论:该方法简便易行,重复性好,准确度高,为丹红片的质量控制奠定了基础.
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| 关 键 词： | 丹红片 丹参素 原儿茶醛 高效液相色谱法 |
| 收稿时间： | 2011/8/28 0:00:00 |
| 修稿时间： | 2011/10/25 0:00:00 |
Determination of Danshensu and Protocatechuic Aldehyde in Danhong Tablets by HPLC |
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| Zhao Yake
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Zhang Changgong
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Long Qing' e
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Zhang Mengjie
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Wang Teng.Determination of Danshensu and Protocatechuic Aldehyde in Danhong Tablets by HPLC[J].China Pharmacist,2012,15(1):68-70. |
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| Authors: | Zhao Yake Zhang Changgong Long Qing' e Zhang Mengjie Wang Teng |
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| Institution: | Department of Pharmacognosy,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Department of Pharmacognosy,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Department of Pharmacognosy,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Department of Pharmacognosy,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Department of Pharmacognosy,School of Pharmacy,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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| Abstract: | Objective： To develop an HPLC method for the determination of Danshensu and protocatechuic aldehyde in Danhong tablets. Method： A DiamonsilTM Cls column （250 mm x4. 6 mm,5 um） was used at 30 ℃ ,the mobile phase consisted of methanol- 0. 05% phosphoric acid aqueous solution （20： 80） ,the flow rate was 1.0 ml-min-1 and the detection wavelength was 280 nm. Result： The tinear ranges were 8. 160-97. 920 mg.L-1 （r =0. 999 3） for sodium Danshensu and 0. 292-2. 336 mg-L-1 （r =0. 999 3）for proto- eatechuic aldehyde. The average recovery of Danshensu and protocatechuie aldehyde was 100. 06% （ RSD = 0. 46% ） and 99. 92% （RSD = 0. 69% ）,respectively （n = 6 ）. Conclusion： The method is simple, accurate and reliable with good reproducibility. It can be used as a quality control method for Danhong tablets. |
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| Keywords: | Danhong tablets Danshensu Protocatechuic aldehyde HPLC |
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