| 怀牛膝中总皂苷含量测定 |
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| 引用本文： | 卫修来,高昌琨,高建,徐先祥.怀牛膝中总皂苷含量测定[J].安徽医药,2007,11(5):424-425. |
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| 作者姓名： | 卫修来 高昌琨 高建 徐先祥 |
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| 作者单位： | 安徽医科大学第一附属医院药剂科,安徽,合肥,230022;安徽中医学院第一附属医院药剂科,安徽,合肥,230038 |
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| 摘 要： | 目的 建立怀牛膝中总皂苷含量测定方法.方法 采用高氯酸-香草醛比色法,其原理是怀牛膝中总皂苷与该显色剂反应后,可在相应波长处(544 nm)进行测量.结果 比色法所测定的线形范围为0.2～1.2 mg,相关系数r=0.998 4.平均回收率96.0%,RSD＝3.23%.结论 本法简便、准确,可用于怀牛膝总皂苷含量测定.
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| 关 键 词： | 怀牛膝 总皂苷 含量测定 |
| 文章编号： | 24370488 |
| 修稿时间： | 12 27 2006 12:00AM |
Determination of total saponins in achyranthes bidentata |
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| WEI Xiu-lai,GAO Chang-kun,GAO Jian,XU Xian-xiang.Determination of total saponins in achyranthes bidentata[J].Anhui Medical and Pharmaceutical Journal,2007,11(5):424-425. |
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| Authors: | WEI Xiu-lai GAO Chang-kun GAO Jian XU Xian-xiang |
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| Institution: | 1. Dept of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei Anhui 230022 ; 2. Dept of Pharmacy, The First Affiliated Hospital of Anhui College of TCM , Hefei Anhui 230038 |
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| Abstract: | Aim To establish the method of determination of total saponins in Achyranthes bidentata.Method Anisaldehyde colormetric method analysis was carried out.The detection wavelength was 544 nm.Result The method was linear at the range of 0.2~1.2 mg,r=0.998 4.The average recovery was 96.0%,and RSD was 3.23%(n=5).Conclusion The method is simple,accurate,and may be used for the quantitative determination of Achyranthes bidentata. |
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| Keywords: | achyranthes bidentata total saponins determination |
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