硒对大鼠急性缺血心肌溶酶体膜的影响

结扎大鼠冠状动脉，造成急性心肌梗塞模型，观察硒对缺血心肌溶酶体酶的影响．结果表明，心肌缺血１小时后，缺血区心肌溶酶体酸性磷酸酶（ＡＣＰ）、组织蛋白酶Ｄ（ＣＤ）的游离酶活性（Ｆ）及游离酶活性／总酶活性比值（Ｆ／Ｔ）均明显升高，ＧＳｌｌ－Ｐｘ活性显著降低，脂质过氧化物含量（ＭＤＡ）明显增加。硒预处理大鼠，心肌急性缺血后．Ｆ及Ｆ／Ｔ显著下降，ＧＳＨ－Ｐｘ明显升高，ＭＤＡ含量显著降低。硒可能通过提高缺血心肌ＧＳＨ－Ｐｘ活性，阻止自由基介导的脂质过氧化，增加溶酶体膜的稳定性，从而减轻心肌缺血性损伤。

Effects or selenium on acute ischemic myocardial lysosomal membrane in rats

The coronary artery of rat was ligated to make the model of myocardial infarction, and the effects of selenium (Se) on ischemic myocardial lysosomal enzymes were observed. The results indicated a significant increase in nonsedimentable activities (F) of myocardial lysosomal acid phosphatase (ACP), cathepsin D(CD) and F/total enzyme activities (T) appeared in ischemic area after an hour's ischemia. Simu taneously, the GSH-Px activity was decreased significantly, and the content of lipid peroxidation product MDA was significantly increased. In the rats pretreated with Se, the F and F/T of ACP, CD and MDA were decreased significantly, and GSH-Px activity was increased significantly. Se may protect against free radical-mediated lipid peroxidation and stabilize the myocardial lysosomal membrane and protect myocardium from iscbemic injury by raising the GSH-Px activity.