氯化甲基汞对大鼠脑神经细胞凋亡及P^53基因表达的影响

选用Ｗｉｓｔａｒ系雄性大鼠，经皮下注射给予１０ｍｇ／Ｋｇ体重氯化甲基汞（ＣＨ３ＨｇＣｌ），连续７天，第１５天取其脑组织，利用细胞和分子生物学技术进行ＤＮＡ电泳和流式细胞术（ＦＣＭ）分析。实验结果，染毒组大鼠脑组织ＤＮＡ电泳呈现特征性梯形电泳带；ＦＣＭ分析染毒组大鼠脑神经细胞的凋亡率、Ｐ５３基因表达率显著高于对照组（Ｐ＜０．０５）。结果表明：ＣＨ３ＨｇＣｌ以诱导凋亡方式导致脑神经细胞损伤，Ｐ５３基因参与ＣＨ３ＨｇＣｌ诱导脑神经细胞凋亡的基因调控过程。

Effects of Methylmercury On Apoptosis Of Brain Neurons From Rats and Expressions of P 53 Gene

Male wistar rats were employed in the present study.They were administered successively 10mg methylmercury(CH 3HgCl)/Kg weight by subcutaneous injection for 7 days.On 15th day the brain tissues of rats were removed and DNA electrophoresis and analysis of flow cytometry were performed by making use of technologis on cellular and molecular biology.DNA was extracted from brain tissues of CH 3HgCl treated rats.A typical ladder of DNA fragments was seen by agarose gel electrophoresis.Neurons apoptosis rate and expression lever of P 53 gene from CH 3HgCl treated group were obviously higher than that from control group by analysis of flow cytometry(P<0 05).The experimental results demonstrate that CH 3HgCl causes injury of brain neurons by inducing apoptosis.P 53 gene participates in gene regulation in process of CH 3HgCl induced apoptosis of brain neurons.