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临床标体中钩端螺旋体及其L型的检测与鉴定
引用本文:宋秀宇,唐素兰,林特夫,黄谷良,汤郡,娄峥,马登宏,张郁文,屈洪党. 临床标体中钩端螺旋体及其L型的检测与鉴定[J]. 中国人兽共患病杂志, 1999, 16(5): 33-35
作者姓名:宋秀宇  唐素兰  林特夫  黄谷良  汤郡  娄峥  马登宏  张郁文  屈洪党
作者单位:蚌埠医学院微生物学教研室!蚌埠,233003,蚌埠医学院微生物学教研室!蚌埠,233003,蚌埠医学院微生物学教研室!蚌埠,233003,蚌埠医学院微生物学教研室!蚌埠,233003,蚌埠医学院微生物学教研室!蚌埠,233003,蚌埠医学院微生物学教研室!蚌埠,233003,蚌埠医学院附院神经内科,蚌埠医学院
摘    要:目的 为探讨钩体 L 型在钩体病患者体内是否存在及是否具有致病作用。方法 对患者血、 C S F 进行 M A T;对患者血、 C S F 及尿液进行钩体及其 L 型培养; 对培养出的钩体 L 型进行免疫酶染色、免疫荧光染色、 S D S- P A G E、 D I B A及电镜等方法鉴定。结果  M A T 阳性率为1917 % (60313) ; 钩体普通培养阳性率1188 % (41345) ; 钩体 L 型阳性率2493 % (86345) 。培养出的 L 型经上述方法鉴定为钩体 L 型。结论 钩体 L 型培养应与 M A T 及钩体普通培养同时进行,以防漏诊。

关 键 词:钩端螺旋体 L型 培养 钩端螺旋体病

DETECTION AND IDENTIFICATION OF LEPTOSPIRA AND ITS L-FORMS IN PATIENTS WITH LEPTOSPIROSIS
Song Xiuyu Tang Sulan Lin Tefu et al. DETECTION AND IDENTIFICATION OF LEPTOSPIRA AND ITS L-FORMS IN PATIENTS WITH LEPTOSPIROSIS[J]. Chinese Journal of Zoonoses, 1999, 16(5): 33-35
Authors:Song Xiuyu Tang Sulan Lin Tefu et al
Abstract:Aim To study whether leptospira L-forms persisted in patients with leptospirosis and how its pathogenicities were.MethodsThe blood and CSF were examined by MAT.The blood,CSF and urine were also cultivated leptospira and its L-forms.The leptospira L-forms were identified with immunocytochemical staining,immunofluorescence,SDS-PAGE,DIBA and electron microscopy.ResultsThe positive rate of MAT was 19 17%.The isolation rats of leptospira and its L-forms were 11 88% and 24 93% respectively.The L-forms were identified as leptospira L-forms by the multiple manners.ConclusionThe culture of leptospia L-forms should be conducted along with MAT and culture of leptospira.
Keywords:Culture of leptospira L-forms Identification MAT
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