Th17细胞在小鼠H22原位肝癌模型中的表达和意义

目的 检测小鼠原位肝癌模型中Th17细胞的表达,探讨其在肝癌免疫中的作用. 方法 30只Balb/C小鼠随机分为肝癌模型组（10只）、生理盐水对照组（10只）、空白对照组（10只）,流式细胞术检测小鼠分离纯化后的各组新鲜脾脏Th17细胞的相对比例;实时RT-PCR检测各组肝组织IL-17基因和RORγT基因表达水平.结果 肝癌模型组脾脏Th17/ CD4＋T细胞的比例高于生理盐水对照组脾脏、空白对照组脾脏,差异有统计学意义（P 〈 0.01）,而各对照组之间的比例差异无统计学意义（P 〉 0.05）.肝癌模型组肿瘤组织IL-17 mRNA和RORγT mRNA的表达水平均高于各自对照组,差异有统计学意义（P 〈 0.01）,而IL-17 mRNA和RORγT mRNA在各自对照组之间表达水平的差异无统计学意义（P 〉 0.05）. 结论 Th17细胞在小鼠原位肝癌模型中数量增多,IL-17表达升高,增强免疫反应,但在肝癌免疫中的作用及机制复杂,有待深入研究.

Expression and its significance of Th17 cells in H22 orthotopic tumor model of hepatocellular carcinoma in mice

Objective To detect the expression of Th17 cells in orthotopic tumor models of hepatocellular carcinoma in mice and investigate the role of it in hepatocellular carcinoma immunization. Methods Thirty Balb/C mice were randomly divided into groups： tumor model of hepatocellular carcinoma group（n -- 10）, normal saline control group （n= 10）,blank group （n=10）. Fresh spleen tissues of mice were separated and purified,then the relative proportion of Thl7 cells was detected by flow cytometry; IL-17 genic expressoion and RORγT genic expressoion of the liver tissues was detected by Realtime PCR. Results Thl7/CD4＋T cells relative proportion of the spleen tissues in the tumor mod- els of hepatocellular carcinoma group was higher than control groups,the differences were significant （P 〈 0.01 ） ,but differences of the relative proportion between the spleen tissues of normal saline control group and blank group were not significant （P 〉 0.05）. IL-17 genic expression and RORγT genic expression of the cancer tissues was higher than the corresponding control groups,the differences were significant （P 〈 0.01 ）,but expression differences of IL-17 genic and RORγT genic among the corresponding control groups including the liver tissues of blank group,the liver tissues of normal saline control group and the liver tissues around cancer were not significant （P 〉 0.05）. Conclusion The number of Th17 cells and expression of IL-17 in orthotopic tumor models of hepatocellular carcinoma in mice increase, of the function to intestify immunological response,but the role and mechanism in hepatocellular carcinoma immunization is complex , need to be studied thorough.