纤支镜毛刷细胞块技术的应用方法和价值

目的探讨纤支镜毛刷细胞块技术的应用方法和价值。方法对32例纤支镜毛刷常规涂片后再将剩余细胞用10％中性福尔马林固定制成细胞蜡块（cellblock，CB），先行常规HE染色，再根据需要选择酶标行免疫组化。结果CB常规HE染色细胞清晰，有一定的组织学结构，有利于明确诊断；免疫组化则阳性定位准确．着色清晰，对肿瘤分型效果较好。两种染色方法染色效果均好于常规涂片，差异有显著统计学意义（P〈0．01），与肺活检及术后组织切片差异无统计学意义（P〉0．05）。结论用10％中性福尔马林固定的纤支镜毛刷CB的制作方法简便、经济、染色效果好，在诊断肺疾病、特别是肺肿瘤中有较高的实用价值和广阔的应用前景。值得推广。

The application methods and value of fiber lens brush cell block technique

Objective To study the application methods and value of fiber lens brush cell block technique. Methods For 32 cases of fiber lens brush after conventional smear in the remaining cells again fixed with 10% formaldehyde wax block made from cells （cell block, CB）, the first routine HE staining, again to choose according to the need to enzyme immunohistochemical marking line. Results CB routine HE staining cells clear, histologic structure to a certain extent, was conducive to clear diagnosis; Immunohistochemical positive for accurate positioning, color clear, has good effect for tumor classification. Two dyeing methods were better than regular smear dyeing effect, so the significant difference of statistically was significant （P 〈 0.01）, and the lung biopsy and postoperative biopsy difference was not significant （P 〉 0.05）. Conclusion The production method of the fiber lens brush CB fixed with 10% of formaldehyde is simple, economic and The dyeing effect is good, in the diagnosis of pulmonary diseases, especially lung cancer has a high practical value and broad application prospect, the production method is worth promoting.