Nano-topology guided neurite outgrowth in PC12 cells is mediated by miRNAs |
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| Affiliation: | 1. Advanced Materials for Micro- and Nano-Systems Programme, Singapore-MIT Alliance, Singapore;2. Department of Biochemistry, National University of Singapore, Singapore;3. Department of Electrical and Computer Engineering, National University of Singapore, Singapore;4. NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore;1. Department of Medical Oncology, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India;2. Department of Pulmonary Critical Care and Sleep Medicine, All India Institute of Medical Sciences, New Delhi, India;3. Department of Surgical Oncology, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India;1. DWI-Leibniz Institute for Interactive Materials, Aachen 52074, Germany;2. Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen 52074, Germany;3. Department of Advanced Materials for Biomedicine, Institute of Applied Medical Engineering, RWTH Aachen University, Aachen 52074, Germany;1. Department of Human Genetics, Saarland University, Building 60, 66421 Homburg/Saar, Germany;2. Clinical Bioinformatics, Saarland University, Building E2.1, 66123 Saarbrücken, Germany;1. Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;2. Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China;3. Department of Laboratory Medicine, the 82nd Hospital of the People’s Liberation Army, Huaian 223001, China;4. Institute of Pediatrics, Nanjing Medical University, Nanjing 210029, China |
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| Abstract: | MicroRNAs (miRNAs) are master regulators of gene expression at post-transcriptional level. The present study investigated the involvement of miRNAs in topological guidance of neurite outgrowth in an NGF treated PC12 cell model cultured on nano-patterned polyethylene terephthalate (PET) substrates fabricated with interference lithography. The expressions of 38 neuronal miRNAs were measured and 3 were found to be differentially regulated during topological guidance of neurite outgrowth. Altering the intracellular levels of these miRNAs disrupted the orderly growth of neurite along nano-patterned substrate. Our results showed miRNAs to be versatile regulators and their involvement should be thoroughly investigated for better understanding of biological processes.From the Clinical EditorIn this basic science study, strong evidence was found that topological guidance is only one factor, and miRNA-s regulate axonal outgrowth from neurites. Nano-patterned polyethylene terephthalate substrates were used for the study, fabricated using interference lithography. Further studies of this biologically relevant process may pave the way to clinically useful axonal regrowth and axonal guidance methods. |
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