lncRNA LOC730101对肝细胞癌增殖、迁移与侵袭的影响

目的 探讨长链非编码RNA(long non-coding RNA,lncRNA)LOC730101对肝细胞癌（hepatocellular carcinoma,HCC）生物学行为的影响。方法 利用R软件分析TCGA数据库LIHC数据集374例HCC组织和50例正常组织中LOC730101的表达差异。收集2018年广西医科大学附属肿瘤医院外科手术切除、经病理确诊的40例HCC患者的癌组织及其相应癌旁组织，培养肝癌细胞系Huh-7、HCCLM3、HepG2和人正常肝细胞系L02，采用qRT-PCR检测组织和细胞系中LOC730101的相对表达水平。在肝癌细胞系Huh-7、HCCLM3中采用慢病毒感染法构建过表达LOC730101(OE-LOC730101)和敲低LOC730101(sh-LOC730101)的稳转细胞株，同时设置相应空白对照（control组）及阴性对照组（sh-NC组、OE-NC组），然后采用qRT-PCR验证感染效果，CCK-8实验检测各组细胞的增殖能力，划痕愈合实验和Transwell小室侵袭实验检测各组细胞的迁移、侵袭能力。结果 TCGA数据库分析结果显示，LOC...

Effect of lncRNA LOC730101 on the proliferation,migration and invasion of hepatocellular carcinoma

Objective To investigate the effect of long non?coding RNA (lncRNA) LOC730101 on the biological behavior of hepatocellular carcinoma (HCC). Methods R software was used to analyze the difference in the expression of LOC730101 in 374 HCC tissues and 50 normal tissues from the LIHC dataset of TCGA. 40 pairs of HCC tissues and corresponding adjacent tissues were collected from the patients with HCC who underwent surgical resection and were pathologically diagnosed in Guangxi Medical University Cancer Hospital in 2018, and the HCC cell lines Huh?7, HCCLM3, HepG2 and human normal liver cell line L02 were cultured. The relative expression level of LOC730101 in tissues and cell lines was detected by qRT?PCR. The stable overexpressing cell lines LOC730101 (OE?LOC730101) and knocking down LOC730101 (sh?LOC730101) were constructed by lentivirus infection in HCC cell lines Huh?7 and HCCLM3, the corresponding control group (control) and negative control groups (sh?NC, OE?NC) was set up at the same time, and qRT?PCR was used to verify the infection effect. The proliferation ability of cells in each group was detected by CCK?8. The migration and invasion ability of cells in each group were detected by wound healing assay and Transwell invasion assay. Results TCGA database analysis showed that the expression level of LOC730101 in HCC tissues was higher than that in normal tissues (P<0.001). The results of qRT?PCR showed that the expression level of LOC730101 in HCC tissues was significantly higher than that in adjacent tissues (P<0.001). The expression level of LOC730101 in liver cancer cell lines Huh?7, HepG2 and HCCLM3 were also higher than that in human normal liver cell line L02 (all P<0.05 ). Compared with the corresponding negative control group, the proliferation, migration and invasion ability of cells were decreased in the sh?LOC730101 group (all P<0.05), while increased in the OE?LOC730101 group (all P<0.01). Conclusions lncRNA LOC730101 is up?regulated in HCC tissues and cells, and may promote its proliferation, migration and invasion.