| 脑缺血后海马CA1区细胞凋亡现象的观察 |
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| 引用本文： | 张惠 曾祥龙. 脑缺血后海马CA1区细胞凋亡现象的观察[J]. 医学争鸣, 1997, 18(3): 263-265 |
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| 作者姓名： | 张惠 曾祥龙 |
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| 作者单位： | 第四军医大学西京医院麻醉科 |
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| 摘 要： | 观察大鼠完全性前脑缺血再灌注损伤过程中海马ＣＡ１区神经元死亡的另一种形式－细胞凋亡。采用琼酯糖凝胶电泳及原位缺口翻译法，观察海马ＣＡ１区神经元是否有凋亡特征性改变。结果；脑缺血损伤后５ｄ，从海马ＣＡ１区神经元提取ＤＮＡ，其琼酯糖凝胶电泳呈现典型梯状结构。而采用原位缺口翻译法，发现缺血损伤后３ｄ，海马ＣＡ１区开始出现胞核染色体阳性的凋亡细胞，缺血损伤后５ｄ，凋亡细胞达到高峰。结论：海马ＣＡ１区尽管
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| 关 键 词： | 脑缺血 细胞凋亡 海马 |
Observation of apoptosis in hippocampus CA1 after cerebral ischemia by biochemistry and moleculabiology |
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| Zhang Hui,Zeng Xianglong,Li Haimin,Zhang Yingmin,Deng Fangxia. Observation of apoptosis in hippocampus CA1 after cerebral ischemia by biochemistry and moleculabiology[J]. Negative, 1997, 18(3): 263-265 |
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| Authors: | Zhang Hui Zeng Xianglong Li Haimin Zhang Yingmin Deng Fangxia |
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| Affiliation: | Zhang Hui,Zeng Xianglong,Li Haimin,Zhang Yingmin,Deng Fangxia Department of Anesthesiology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033 |
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| Abstract: | Objective: To observe another form of cell death (apoptosis) in the rat hippocampus CA1 pyramidal neurons following forebrain ischemia. Methods: Electrophoresis in agarose gels and in situ nick translation were employed to observe whether neurons in hippocampus CA1 appeared characteristic alteration of apoptosis. Results: DNA was extracted from hippocampus CA1 neurons 5 d after ischemic injury, which exhibited the characteristic ladder pattern in agarose gels. Using INT, positive staining was occasionally seen in nuclei of the hippocampus CA1 neurons 3 d after ischemic injury; apoptotic neurons reached a maximum 5 d after ischemic injury. Conclusions: Apoptotic mechanism may play an important role in the delayed death of hippocampus CA1 neurons. It may provide novel therapeutic approaches for prevention of cerebral ischemic injury. |
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| Keywords: | cerebral ischemia reperfusion injury apoptosis |
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