Preprotachykinin gene expression in the mediobasal hypothalamus of estrogen-treated and ovariectomized control rats |
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| Authors: | G. J. Romano T. I. Bonner D. W. Pfaff |
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| Affiliation: | (1) Rockefeller University, 1230 York Ave., 10021 New York, NY, USA;(2) Laboratory of Cell Biology, National Institute of Mental Health, 20205 Bethesda, MD, USA;(3) Present address: School of Medicine, The Johns Hopkins University, 725 North Wolfe Street, 21205 Baltimore, MD, USA |
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| Abstract: | Summary We have determined the localization of preprotachykinin (PPT) mRNA-containing neurons in the mediobasal hypothalamus of the rat. PPT mRNA-containing neurons are present in the ventromedial nucleus (with a concentration in the ventrolateral aspect (VL-VM)), the dorsomedial nucleus, the lateral hypothalamus and the arcuate nucleus. This distribution is consistent with the findings of immunocytochemical studies of substance P-immunoreactive neurons in the hypothalamus. We have also examined whether PPT gene expression is regulated by estrogen in the VL-VM by comparing the levels of PPT mRNA in the VL-VM of ovariectomized rats and ovariectomized, estrogen-replaced rats. Both in situ hybridization and slot blot hybridization analysis revealed no changes in PPT mRNA content in the VL-VM following estrogen treatment. These results suggest that estrogen does not regulate lordosis behavior by affecting PPT gene expression in VL-VM neurons. |
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| Keywords: | Preprotachykinin gene Hypothalamus estrogen Ovariectomized rats |
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