细胞生长液与粘附胶原对大肠肿瘤细胞体外生长的影响

本文以ＲＰＭＩ１６４０培养液为基础，探讨了补加因子及粘附胶原对大肠癌细胞体生长和贴壁的影响，结果表明：在基础培养液中添加１０％的补充液可显著促进大肠癌细胞的生长，降低癌细胞对培养液的需求量；并表明，鼠尾胶原可明显促进癌细胞克隆形成率。对于组织贴壁培养，粘附胶原组成功率（６６．７％），亦明显比无胶原被复组（４１．７％）为高。

EFFECT OF CELL MEDIA AND ADHESIVE COLLAGEN ON CELL GROWTH OF LARGE BOWEL CANCERS IN VITRO

In order to improve the sucessive rate of primary culture and sub-culture of large intestinal tumors, supplements were added into RPMI 1640 culture me-dium and collagen substrate was used to improve the anchorage condition. The supple-ments included epithermal growth factor, pentagastrin, transferrin, etc, and collagen sub-strate was prepared from rat tails. The results showed that cell growth was improvedobviously in PRMI 1640 contained 10%supplement media. though it was not promotedwith increase of the concentration of supplements. This supplement media could alsodecrease largely the requirement of cell growth in vitro for serum, which may be help-ful to delav the growth of fibroblast in primary culture. Though there were no relation-ships between plate colonyforming rate and established cancer cell lines, the existanceof adhesive collagen could increase the colony-forming rate of primary culture and sub-culture. The survival rate of primary explant culture in condition of collagen substance(66.7%)was higher than that of the group without collagen coated(41.7%). It is sugges-ted that optimization of culture condition be an important way to Inhance the successfulrate of primary culture and subculture for large bowel tumors.