| VP-16诱导PC-3细胞凋亡和bcl-2及c-myc基因表达的研究 |
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| 引用本文： | 李登欣,董素芹,张建业,张莲英,何维敬.VP-16诱导PC-3细胞凋亡和bcl-2及c-myc基因表达的研究[J].潍坊医学院学报,2000(3). |
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| 作者姓名： | 李登欣 董素芹 张建业 张莲英 何维敬 |
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| 作者单位： | 潍坊医学院附属潍坊市人民医院!山东潍坊261042(李登欣，董素芹)，山东医科大学生化教研室(张建业，张莲英，何维敬) |
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| 摘 要： | 目的 研究 VP- 1 6诱导 PC- 3细胞凋亡和癌基因 bcl- 2及 C- myc表达的关系。方法 用末端标记法(TUNEL )和流式细胞术 (FCM)研究 PC- 3细胞凋亡 ,以免疫组化方法研究 bcl- 2及 C- myc蛋白的表达。结果 TUNEL和FCM检测表明 ,PC- 3细胞的凋亡率随 VP- 1 6作用浓度增加和作用时间延长而升高 ;免疫组化结果显示 :bcl- 2和 c- myc基因表达的阳性百分率随 VP- 1 6作用浓度增加和作用时间延长而降低。结论 VP- 1 6能够诱导 PC- 3细胞凋亡和抑制其bcl- 2和 c- myc基因的表达 ,且具有作用浓度和作用时间依赖性。
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| 关 键 词： | 细胞凋亡 PC-3细胞 VP-16(鬼臼乙叉甙) bcl-2基因 c-myc基因 |
Apoptosis Induced by Etoposide in PC-3 Cells with bcl-2 and c myc Oncogene Expression |
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| LI Deng xin,DONG Su qin,ZHANG Jian ye ,et al.Apoptosis Induced by Etoposide in PC-3 Cells with bcl-2 and c myc Oncogene Expression[J].Journal of Weifang Medical College,2000(3). |
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| Authors: | LI Deng xin DONG Su qin ZHANG Jian ye |
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| Institution: | LI Deng xin,DONG Su qin,ZHANG Jian ye *,et al |
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| Abstract: | Objetive To study the relationship between apoptosis induced by etoposide in PC 3 cells and the expression of bcl 2 and c myc oncogene.Method TdT mediated x dUTP nick end labeling and flow cytometry were emploied to investigate the apoptosis;c myc and bcl 2 protein were detected immunohisto chemically.Result The findings indicated that the percentage of apoptosis cells was increased and the percentage of positive cells of bcl 2 and c myc expression was decreased with the increasing of concentration and exposure time of VP 16.Conclusion VP 16 can induce the programmed cell death of PC 3 cells and inhibit the expression of bcl 2 and c myc,and the inhibition is dose dependent and time dependent. |
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| Keywords: | Apoptosis PC-3 cells VP-16(Etoposide) bcl-2 gene c myc gene |
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