PCR法检测HBVDNA及其与乙肝病毒标志物关系探讨

采用聚合酶链反应 (PCR)法检测 2 56例乙型肝炎患者血清中乙肝病毒 DNA(HBVDNA) ,同时用 EL ISA法检测乙肝病毒标志物 ,即 HBs Ag,抗 - HBs、抗 - HBc、HBe Ag、抗 -HBe。结果表明 HBVDNA的检出率与肝病临床类型无明显关系 ,而与乙肝病毒标志物的存在状态有关。乙肝五项病毒标志物不同组合状态 ,血清 HBVDNA检出率不同 ,以 HBs Ag(+)、抗 - HBc(+)、HBe Ag(+)的组合形式检出率最高 (94 .34% ) ,乙肝病毒标志物中出现抗体及乙肝病毒标志物全阴者仍有 HBVDNA检出

Study on the relationship between DNA of hepatitis B virus and markers of hepatitis B virus

DNA of hepatitis B virus(HBV) in blood serum was detected with polymerase chain reaction (PCR) in 256 cases with hepatitis B,and in those patients,whose five markers of HBV(including HBsAg,anti HBsAg,anti HBc,HBeAg,anti HBeAg) were detected with enzyme linked immunosorbent assay(ELISA).The results showed that the detecting rate of DNA of HBV was not related to the clinical type of hepatitis B,but was well related to present situation of markers of HBV.The detecting rate of serum DNA of HBV was the highest in patients with HBsAg(+),and anti HBc(+),and HBeAg(+).DNAs of HBV were all detected in patients so that the antibody in markers of HBV was positive,or in patients whose markers were all negative.We considered that the results that DNA of HBV was detected with PCR reflected correctly and directly the replication of HBV in patients with hepatitis B.