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| 奥曲肽对人胆囊癌细胞VEGF表达的影响 | |
| 引用本文： | 王菁华,贾晓青,薛宏伟,刘晓峰,袁孟彪. 奥曲肽对人胆囊癌细胞VEGF表达的影响[J]. 山东大学学报(医学版), 2005, 43(2): 117-120 |
| 作者姓名： | 王菁华 贾晓青 薛宏伟 刘晓峰 袁孟彪 |
| 作者单位： | 1. 北京医院加强监护病房,北京,100730 2. 山东大学齐鲁医院消化内科,山东,济南,250012 3. 青岛大学医学院附属医院肿瘤科,山东,青岛,266000 |
| 摘 要： | 目的：探讨奥曲肽在体外对人胆囊癌细胞VEGF表达的影响。方法：体外培养人胆囊癌细胞株GBC—SD，用RT-PCR法检测VEGFmRNA水平，用ELISA法检测培养液上清中的VEGF蛋白水平。结果：GBC-SD高水平表达VEGF；经奥曲肽作用后，VEGFmRNA／β-actin相对系数显著下降，呈剂量依赖效应；奥曲肽也显著降低培养上清液中的VEGF浓度，抑制作用随时间延长和奥曲肽剂量增加而增大。结论：奥曲肽能下调GBC—SD的VEGF的mRNA和蛋白表达水平。 |
| 关 键 词： | 胆囊肿瘤 奥曲肽 内皮，血管 内皮生长因子 |
| 文章编号： | 1671-7554(2005)02-0117-04 |
| 修稿时间： | 2004-05-09 |
Octreotide downregulates the expression of VEGF gallbladder cancer cells |
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| WANG Jing-hua,JIA Xiao-qing,XUE Hong-wei,LIU Xiao-feng,YUAN Meng-biao. Octreotide downregulates the expression of VEGF gallbladder cancer cells[J]. Journal of Shandong University:Health Sciences, 2005, 43(2): 117-120 | |
| Authors: | WANG Jing-hua JIA Xiao-qing XUE Hong-wei LIU Xiao-feng YUAN Meng-biao |
| Affiliation: | WANG Jing-hua1,JIA Xiao-qing2,XUE Hong-wei3,LIU Xiao-feng2,YUAN Meng-biao2 |
| Abstract: | Objective: To investigate whether octreotide can inhibit the vascular endothelial growth factor (VEGF) expression of human gallbladder cancer cells in vitro. Methods: A human gallbladder cancer cell line, GBC-SD, was cultured in vitro. VEGF mRNA was measured quantitatively by RT-PCR method, and VEGF protein concentration in the culture supernatant by enzyme linked immunoabsorbent assay. Results: Both VEGF mRNA and protein were detected in high levels in GBC-SD cells. In 36 hours the concentration of VEGF continuously increased. Octreotide significantly decreased the expression of VEGF mRNA and protein, with dose and time-related effects. Conclusion: Octreotide downregulates the expression of VEGF in gallbladder cancer cells in vitro. |
| Keywords: | Gallbladder neoplasms Octreotide Endothelium vascular Endothelial growth factor |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |