腹部术后疲劳综合征大鼠模型的建立及其评价

目的 建立和评价腹部术后疲劳综合征大鼠模型。方法采用手术切除大鼠部分肝脏，术后观察动物的一般情况、鼠尾悬挂实验、负重游泳力竭实验，血清蛋白，转铁蛋白、血清铁，小肠黏膜损伤病理评价，肝脏白蛋白基因表达等指标。结果70％肝切除实验大鼠一般情况差、体力耐受力下降，有一定程度的抑郁表现，并在营养学指标、小肠黏膜应激反应损伤和肝清蛋白基因表达等方面发生明显改变。结论切除70％肝脏制作大鼠动物模型，具有临床术后疲劳综合征的基本特征，可作为研究腹部手术后疲劳综合征实验动物模型。

Establishment and assessment of a model of abdominal postoperative fatigue syndrome in rats

Objective To establish and assess a model of abdominal postoperative fatigue syndrome (POFS) in rats. Methods After 70% hepatectomy was performed, the following observations of the animals were made:general condition, rat tail suspension test,weight carrying swim fatigue test,serum levels of albumin,ferrition,and iron,pathologic assessment of injury of small intestinal mucosa and hepatic albumin gene expression .Results After 70% hepatectomy of the rats,their general candition was poor,the level of physical tolerance decreased,they showed a certain amount of depression,and marked changes were found in nutritional index,stress injury of small intestinal mucosa and hepatic albumin gene expression.Conclusions A 70% hepatectomy rat model has the basic characteristics of clinical abdominal POFS, and can be used as an experimental animal model for the study of abdominal POFS.