复方甘草酸苷对外周血单核细胞趋化因子表达的影响

目的:探讨复方甘草酸苷(SNMC)是否可以通过抑制外周血分离单核细胞(PBMC)趋化因子的表达而对变态反应性疾病发挥治疗作用。方法:抽取15名健康人外周血并获得PBMC,将PBMC分为SNMC、地塞米松、咪唑斯汀、氯雷他定4个药物组,并设加入及不加入致炎刺激物的阳性对照组和阴性对照组,采用荧光定量聚合酶链反应测定PBMC中趋化因子RANTES、MCP-1、MIP-1α mRNA的表达,酶联免疫吸附法测定上清液中上述趋化因子的含量。结果:阳性对照组3种趋化因子的mRNA表达和含量明显升高,各药物组明显低于阳性对照组,其中MIP-1α mRNA表达地塞米松和SNMC组与阳性对照组比较具有显著性差异(P<0.05)。结论:SNMC可能通过抑制PBMC分泌MCP-1、RANTES、MIP-1α等趋化因子而对变态反应性疾病发挥治疗作用。

Effects of Compound Glycyrrhizin on Chemotactic Factors＇ Expression in Peripheral Blood Mononuclear Cells

OBJECTIVE： To study whether the therapeutic action of compound glycyrrhizin （SNMC） on allergic disorders can be achieved through inhibition on the expression of chemotactic factors in peripheral blood mononuclear cells （PBMC） . METHODS： PBMC of 15 healthy subjects were obtained and divided into 4 drug-treated groups： SNMC group, dexamethasone group, mizolastine group, and loratadine group; meanwhile, a positive control group （proinflammatory stimulant was added） and a negative control group （no proinflammatory stimulant was added） were used as control. Fluorescent quantitation polymerase chain reaction was employed to determine the mRNA expression of the chemotatic factors including RANTES, MCP-1, MIP la and ELISA was employed to detect their concentrations in supernatant. RESULTS： In the positive control group, both the mRNA expressions and the concentrations of the 3 chemotatic factors increased significantly, and which were significantly lower in the drug - treatment groups than in the positive control group. There were significant differences between the positive control group and dexamethasone - treated group or SNMC - treated group in the expression of MIP-1α mRNA （P 〈 0.05）. CONCLUSION： The therapeutic action of SNMC on allergic disorders is possibly achieved by inhibiting secretion of chemotactic factors such as RANTES, MCP-1 and MIP-1α etc in peripheral blood mononuclear cells.