| HPLC法测定皂角刺提取物中槲皮素的含量 |
| |
| 引用本文： | 田吉,;何兵,;李翅翔,;肖顺汉.HPLC法测定皂角刺提取物中槲皮素的含量[J].中国药房,2009(15):1156-1157. |
| |
| 作者姓名： | 田吉 ;何兵 ;李翅翔 ;肖顺汉 |
| |
| 作者单位： | [1]泸州医学院药学院药物研究所,泸州市646000; [2]泸州医学院药学院,泸州市646000 |
| |
| 基金项目： | 四川省教育厅重点项目（2005A076） |
| |
| 摘 要： | 目的:建立以高效液相色谱法测定皂角刺提取物中槲皮素含量的方法。方法:色谱柱为Dikma Kromasil C18(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(30∶70),检测波长为360nm,柱温为30℃,流速为1.0mL·min-1。结果:槲皮素进样量在0.134～1.072μg范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为98.98%,RSD=1.87%(n=9)。结论:本方法灵敏、准确、简便、快速,可用于皂角刺提取物的质量控制。
|
| 关 键 词： | 皂角刺 提取物 槲皮素 含量测定 高效液相色谱法 |
Determination of Quercetin in Extracts of Gleditsia sinensis by HPLC |
| |
| Institution: | TIAN Ji, HE Bing, XIAO Shun-han,LI Chi-xiang(Institute of Materia Medica, School of Pharmacy of Luzhou Medical College, Luzhou 646000, China;School of Pharmacy of Luzhou Medical College, Luzhou 646000, China) |
| |
| Abstract: | OBJECTIVE： To establish an HPLC method for the determination of quercetin in the extracts of Gleditsia sinensis .METHODS： The chromatographic separation was performed on Kromasil C18 column（250mm×4.6mm,5μm） with column temperature kept at 30 ℃ .The mobile phase consisted of acetonitrile - 0.1% phosphoric acid solution（30 ： 70） at a flow rate of 1.0 mL · min^-1 with detection wavelength set at 360 nm. RESULTS： The linear range of quercetin was 0.134--1.072 μg（r = 0.999 9） with its average recovery at 98.98% （RSD = 1.87%, n = 9） . CONCLUSION： The established method was proved to sensitive, accurate, simple, rapid and applicable for the quality control of the extracts of Gleditsia sinensis. |
| |
| Keywords: | Gleditsia sinensis Extracts Quereetin Content determination HPLC |
| 本文献已被 维普 等数据库收录！ |
|
