HPLC法测定癣净颗粒中苦参碱、氧化苦参碱的含量

目的:建立以高效液相色谱法测定癣净颗粒中苦参碱、氧化苦参碱含量的方法。方法:色谱柱为TikeginNH2氨基键合硅胶柱(150mm×4.6mm,5μm),流动相为乙腈-无水乙醇-水(80∶10∶10),检测波长为220nm。结果:苦参碱、氧化苦参碱的进样量分别在0.0845～0.845μg(r=0.9999)、0.048～0.48μg(r=0.9995)范围内与各自峰面积积分值呈良好的线性关系;苦参碱、氧化苦参碱的平均回收率分别为99.30%、97.35%,RSD分别为1.63%(n=6)、1.98%(n=6)。结论:本方法准确、简便,可用于癣净颗粒的质量控制。

Determination of Matrine and Oxymatrine in Xuanjing Granules by HPLC

OBJECTIVE： To determine the contents of matrine and oxymatrine in Xuanjing granules by HPLC. METHODS：The samples were separated on Tikegin NH2 amino bonded silica gel column（ 150 mm×4.6 ram, 5 μm） with the mobile phase consisted of acetonitrile- dehydrated alcohol- water （80 ： 10 ： 10）under an detection wavelength of 220 nm.RESULTS： Te linear ranges of matrine and oxymatrine were 0.084 5-0. 845μg（ r = 0. 999 9） and 0.048-0.48 μg（ r = 0.999 5）, respectively, with their average recoveries at 99.30% and 97.35%, respectively and RSD at 1.63% （n = 6） and 1.98% （n = 6）,respectively. CONCLUSION： This method was proved to be accurate, simple and applicable for the quality control of Xuanjing granules.