| HPLC法测定尼莫地平脂肪乳中主药的含量 |
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| 引用本文： | 俞发,;马守栋,;李明春,;李晓,;李友伟,;杨学平. HPLC法测定尼莫地平脂肪乳中主药的含量[J]. 中国药房, 2009, 0(19): 1505-1506 |
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| 作者姓名： | 俞发, 马守栋, 李明春, 李晓, 李友伟, 杨学平 |
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| 作者单位： | [1]解放军第458医院药剂科,广州市510515; [2]解放军第401医院药剂科,青岛市266071 |
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| 摘 要： | 目的:建立以高效液相色谱法测定尼莫地平脂肪乳中尼莫地平含量的方法。方法:色谱柱为Lichrospher C18,流动相为甲醇-水-四氢呋喃(38∶29∶29),流速为1.3mL.min-1,检测波长为237nm,柱温为39℃,进样量为20μL。结果:尼莫地平检测浓度的线性范围为1.0~40.0μg.mL-1(r=0.999 8);平均回收率为98.62%,RSD=1.28%。结论:本方法可用于该制剂的含量测定。
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| 关 键 词： | 高效液相色谱法 尼莫地平 脂肪乳 含量测定 |
Determination of Main Component in Nimodipine Emulsion by HPLC |
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| Affiliation: | YU Fa, MA Shou-dong, LI Ming-chun, LI Xiao, LI You-wei, YANG Xue-ping(1.Dept. of Pharmacy, No. 458 Hospital of the PLA, Guangzhou 510515, China;2.Dept. of Pharmacy, No. 401 Hospital of the PLA, Qingdao 266071, China) |
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| Abstract: | OBJECTIVE： To establish an HPLC method for the determination of the content of nimodipine in nimodipine emulsion. METHODS： The sample was separated on Lichrospher C18 column with methanol- water- tetrahydrofuran （38 ： 29 ： 29） as the mobile phase at a flow rate of 1.3 mL · min ^-1. The detection wavelength was set at 237 nm; The column temperature was set at 39 12 and the sample size was 20μL. RESULTS： The linear range of nimodipine was 1.0--40.0μg · mL^-1 （r = 0.999 8） and the average recovery rate was 98.62% （RSD = 1.28%） . CONCLUSION： The established HPLC method is applicable for the determination of nimodipine content in nimodipine emulsion. |
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| Keywords: | HPLC Nimodipine Emulsion Content determination |
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