An improved method of analysing 2,5-hexanedione in urine |
| |
| Authors: | L Perbellini D M Marhuenda Amoros A C Cardona Llorens C Giuliari F Brugnone |
| |
| Affiliation: | Institute of Occupation Medicine, University of Verona, Policlinico Borgo Roma, Italy. |
| |
| Abstract: | A short gas-chromatographic method for analysing urinary concentrations of 2,5-hexanedione is based on acid hydrolysis of urine at pH below 0.1 and "purification" of the urine samples by microcolumns containing an octadecyl-silane phase. A 5% acetonitrile solution allows a fairly selective elution of 2,5-hexanedione from the microcolumns. Recovery of 2,5-hexanedione from urine is as great as 79.9%. The variation coefficient of the measurements is 2.8%. The results obtained from different working conditions and using packed or wide bore or capillary gas-chromatographic columns are reported. |
| |
| Keywords: | |
|
|
