血红蛋白与转铁蛋白联合检测粪便隐血

目的探讨邻联甲苯胺、血红蛋白胶体金检测试纸（Hb）、转铁蛋白胶体金检测试纸（Tf）同时检测粪便潜血对诊断消化道出血性疾病的临床价值。方法对100例健康人及152例有消化道出血疾病的患者粪便标本，用邻联甲苯胺、血红蛋白胶体金诊断试纸、转铁蛋白胶体金诊断试纸检测粪便隐血。结果100例健康人邻联甲苯胺阳性检出率为12％，血红蛋白阳性检出率为2．0％，转铁蛋白阳性检出率为2．0％，同时检测粪便中血红蛋白、转铁蛋白阳性检出率为2．0％；152例有消化道出血疾病的患者邻甲苯胺阳性检出率为69．5％，血红蛋白阳性检出率为73．0％，转铁蛋白阳性检出率为88．2％，同时检测粪便中血红蛋白、转铁蛋白阳性检出率为91．2％。结论免疫法灵敏度高、特异性强，但是血红蛋白由于受到肠内细菌的作用及大肠粘膜产生的粘液成分影响而变性，出现假阴性反应。因此同时测定血红蛋白和转铁蛋白，可提高消化道出血的阳性检出率。

Clinical application of fecal occult blood testing of hemoglobin and transferrin at the same time

Objective To discuss the clinical value when using o-aniline, colloidal gold detection paper hemoglobin (Hb),transferrin colloidal gold detection paper (Tf) at the same time for fecal occult blood test to diagnose diseases of the gastrointestinal disease bleeding. Methods On 100 cases of healthy people specimens, with o-aniline, hemoglobin of colloidal gold paper, transferrin colloidal gold diagnostic test paper fecal occult blood test. Results Of 100 healthy people o-aniline positive rate of 12%, hemoglobin positive rate of 2.0%,transferrin positive rate of 2.0%, while the detection of hemoglobin and transferrin in the stool, positive rate is2.0%:152 cases of the disease have gastrointestinal bleeding in patients with o-toluidine positive rate of 69.5%,hemoglobin positive rates of 73.0%, transferrin positive rate of 88.2 %, while the detection of hemoglobin and transferrin in the stool, positive rate is 91.2%. Conclusions Immunity assay has high sensitivity and specificity. But hemoglobin due to the role of intestinal bacteria and the intestinal mucus composition of the infection and then cause variability, it can cause a false negative response. So use hemoglobin and transferring to mensurate gastrointestinal bleeding at the same time may increase the positive rate.