T淋巴细胞亚群检测在腹水鉴别诊断中的价值

目的 :探讨T淋巴细胞亚群检测在腹水鉴别诊断中的价值。方法 :对 92例腹水患者 ,其中结核性腹膜炎腹水 38例 ,肝硬化腹水 2 9例 ,癌性腹水 2 5例 ,用流式细胞仪测定其腹水T淋巴细胞亚群。结果 :腹水中T淋巴细胞 (CD 3+ )、T辅助 /诱导细胞亚群 (CD4+ )、T辅助细胞亚群 /T抑制细胞亚群 (CD 4+ /CD 8+ )由高到低依次为结核性腹膜炎腹水 >癌性腹水 >肝硬化腹水 (P <0 .0 1 ) ;CD 8+ 由高到低依次为癌性腹水 >结核性腹膜炎腹水 >肝硬化腹水 (P <0 .0 1 )。结论 :T淋巴细胞亚群在结核性腹膜炎、肝硬化及癌性腹水中存在明显差异。腹水T淋巴细胞亚群检测可用于腹水的鉴别诊断。

The Value of T Lymphocyte Subsets Determination in Ascitic Fluid in the Differential Diagnosis of Ascites

Objective:To investigate the value of T lymphocyte subsets determination in ascitic fluid in the differential diagnosis of ascites.Methods:In 92 cases with ascites,including 38 tuberculous peritonitis,29 liver cirrhosis and 25 carcinomatous ascites,T lymphocyte subsets in ascitic fluid were detected by flow cytometer.Results:T lymphocyte (CD 3 +), helper/inducer T lymphocyte (CD 4 +) and helper T lymphocyte/suppressor T lymphocyte (CD 4 +/CD 8 +) in ascitic fluid,in tuberculous peritonitis,carcinomatous ascites,liver cirrhosis descended gradually (P<0.01).CD 8 + in carcinomatous ascites,tuberculous peritonitis,liver cirrhosis descerded graduaaly (P<0.01).Conclusion:T lymphocyte subsets in ascitic fluid exists obvious difference among the patients with tuberculous peritonitis, liver cirrhosis and carcinomatous ascites.T lymphocyte subsets determination in ascitic fluid can be used in the differential diagnosis of ascites.