| RhD阴性血型抗原分析在临床输血安全中的应用研究 |
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| 引用本文： | 陈荣仓,陈筱华,林碧,孔令光,黄国永,刘保林,甄伟春,林杰. RhD阴性血型抗原分析在临床输血安全中的应用研究[J]. 上海医学检验杂志, 2011, 0(2) |
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| 作者姓名： | 陈荣仓 陈筱华 林碧 孔令光 黄国永 刘保林 甄伟春 林杰 |
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| 作者单位： | 温州市中心血站; |
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| 基金项目： | 温州市卫生局项目(2009B075) |
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| 摘 要： | 目的从临床输血安全的研究角度,实施有效的管理和应用,指导对RhD阴性血型稀有血液资源的保存与实用性稀有血型库的建立。方法采用瓷板法对RhD抗原进行初筛,RhD初筛阴性的血液标本,用间接抗人球蛋白法(IAT)进行确认试验;RhD确认阴性的试验标本再进行吸收和放散试验;再用盐水试管法对RhC、Rhc、RhE和Rhe抗原的鉴定。结果从165 041名献血者初筛出651名RhD抗原阴性者,其中RhD阴性抗原确认为633名,弱D型为18名,在633名RhD抗原阴性献血者中,ABO血型分布为:O型(36.81%)(A型(30.49%)>B型(26.07%)(AB型(6.63%);RhD阴性血型抗原表现型以ccdee(59.08%)和Ccdee(30.96%)居多,其次为CCdee、ccdEe、CcdEe、CCdEe、CcdEE和ccdEE表现型,未见CCdEE表现型。从整体随机抽取确认为阴性的316名献血者中共检测出DEL型71名,所占比例为22.47%。结论把O型CCdEE和ccdEE表现型红细胞,在工作中发现后及时冻存并在临床上针对使用,在建立使用型稀有血型库和解决临床对特殊血型的需求具有实际的临床应用意义。
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| 关 键 词： | RhD阴性 血型抗原 应用 |
Application research of antigen analysis on RhD-negative blood type in the clinical transfusion safety |
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| CHEN Rongcang,CHEN Xiaohua,LIN Bi,KONG Lingguang,HUANG Guoyong,LIU Baolin,ZHEN Weichun,LIN Jie.. Application research of antigen analysis on RhD-negative blood type in the clinical transfusion safety[J]. Shanghai Journal of Medical Laboratory Sciences, 2011, 0(2) |
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| Authors: | CHEN Rongcang CHEN Xiaohua LIN Bi KONG Lingguang HUANG Guoyong LIU Baolin ZHEN Weichun LIN Jie. |
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| Affiliation: | CHEN Rongcang,CHEN Xiaohua,LIN Bi,KONG Lingguang,HUANG Guoyong,LIU Baolin,ZHEN Weichun,LIN Jie.(Central Blood Station of Wenzhou,Zhejiang Wenzhou 325027,China) |
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| Abstract: | Objective To establish the effective management and application guideline for the preservation of RhD-negative blood type scarce resource and the practicality of rare blood bank from the view of blood transfusion safety.Methods By porcelain plate method for screening of RhD antigen,RhD-negative blood samples were detected.The indirect anti-human-globulin test(IAT) was the confirmation test.The RhD-negative specimens were determined by absorb and diffuse test further.RhC,Rhc,RhE and Rhe antigens were identif... |
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| Keywords: | RhD-negative Blood type antigen Application |
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