| 玻璃毛细柱色谱法对脂肪酸的分析 |
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| 引用本文： | 周英田,赵志杰. 玻璃毛细柱色谱法对脂肪酸的分析[J]. 营养学报, 1985, 0(1) |
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| 作者姓名： | 周英田 赵志杰 |
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| 作者单位： | 军事医学科学院军队卫生研究所 天津(周英田)，军事医学科学院军队卫生研究所 天津(赵志杰) |
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| 摘 要： | 通过色谱条件、斜率灵敏度、尾吹气流等的选择,我们建立了一个分析脂肪酸的气相色谱法。脂肪酸甲基化后,在涂交联甲基硅油的毛细柱内的保留时间和碳原子数目密切相关。如以相对保留时间为纵坐标,碳原子数为横坐标,可得一条光滑曲线。方法重现性好,相对标准偏差0.4-1.5％。用以分析六种植物油的脂肪酸组成,结果接近加拿大谷物协会的报告。用于细菌游离脂A的分析,结果与薄层层析和化学分析相一致。
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| 关 键 词： | 玻璃毛细柱气相色谱法 脂肪酸 |
THE GLASS CAPILLARY GAS CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF FATTY ACIDS |
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| Zhou Yingtian,Zhao Zhijie. THE GLASS CAPILLARY GAS CHROMATOGRAPHIC METHOD FOR THE ANALYSIS OF FATTY ACIDS[J]. Acta Nutrimenta Sinica, 1985, 0(1) |
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| Authors: | Zhou Yingtian Zhao Zhijie |
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| Abstract: | A gas chromatographic method was established for the analysis of fatty acid composition of oils. After methlation of the samples, the retention time of the fatty acid esters in the glass capillary coated with crosslin-ked methyl silicone was closely related to the number of carbon atoms.The reproducibity of this method was satisfactory. The relative standard deviation was 0.4-1.5%. Six kinds of vegetable oils were analyzed by this method, and the results were consistent with those reported by Canadian Cereal Association. For analysis of the free fatty acid composition of becte-ria, the results were comparable with those obtained from thin layer chroma-tography and chemical analysis. |
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| Keywords: | glass capillary gas chromatography fatty acid |
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