高龋及无龋儿童变形链球菌分离株的蔗糖粘附能力比较

目的:探讨高龋和无龋儿童变形链球菌(变链菌)临床分离株的蔗糖依赖性粘附能力。方法:选取本实验室从10例高龋儿童和10例无龋儿童(3￣5岁)牙菌斑内分离、鉴定所得的60株变链菌临床分离株进行研究。采用紫外分光光度计,检测来自高龋儿童(dmfs≥6)的39株和无龋儿童(dmfs=0)的21株变链菌在含糖培养基中对玻壁的粘附情况。采用SPSS12.0软件包进行单因素方差分析,比较高龋儿童和无龋儿童之间牙菌斑内变链菌分离株的玻壁粘附能力。结果:在含1%蔗糖的培养基中,高龋组变链菌分离株对玻壁的粘附比平均值为(55.49+26.16)%,无龋组变形链球菌分离株对玻壁的粘附比平均值为(27.01+18.39)%,2组间差异具有显著性,P<0.01。结论:在含蔗糖环境中,分离自高龋儿童牙菌斑的变链菌株对玻壁的粘附能力高于来自无龋儿童的变链菌分离株,提示变链菌临床分离株的粘附能力与其致龋力相关。

Comparison of the sucrose dependent cell adherence of Streptococcus mutans isolates from caries-active and caries-free children

PURPOSE: To study the sucrose dependent cell adhesive ability of Streptococcus mutans islolated from the caries-active and caries-free children. METHODS: 60 isolated Streptococcus mutans strains were selected and identified from the dental plaque of 10 caries-active children and 10 caries-free children (3 to 5 years), in which 39 strains were from caries-active group(dmfs>or=6) and 21 strains from caries-free group(dmfs=0). With the use of ultraviolet spectrophotometer, the sucrose dependent cell adherence to glass wall of the sucrose-containing testing tubes was analyzed. One-way ANOVA was used by SPSS12.0 software package to determine the statistical difference of the adhesive ability between the two groups. RESULTS: The average adhesive ratio of the Streptococcus mutans strains isolated from caries-active group was 55.49%+26.16% in the 1% sucrose-containing culture medium, while the average adhesive ratio of the Streptococcus mutans strains isolated from caries-free group was 27.01%+18.39%. The difference between the two groups was statistically significant (P<0.01). CONCLUSION: In the sucrose-containing circumstance, the sucrose dependent cell adhesive ability of the Streptococcus mutans isolated from the caries-active children was significantly higher than that from the caries-free children. This indicated that the adhesive ability may be related to the caries-causing tendency.