MVPlex assay for direct detection of methicillin-resistant Staphylococcus aureus in naris and other swab specimens |
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Authors: | Podzorski Raymond P Li Haijing Han Jian Tang Yi-Wei |
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Institution: | Department of Pathology, Waukesha Memorial Hospital, Waukesha, Wisconsin 53188, USA. raymond.podzorski@phci.org |
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Abstract: | We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant Staphylococcus aureus (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the “gold standard,” the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% (P = 0.002) and specificities of 95.8% and 98.6% (P < 0.05), respectively. |
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